Human embryonic stem cell-derived oligodendrocyte progenitor cells express the serotonin receptor and are susceptible to JC virus infection
Human embryonic stem cell-derived oligodendrocyte progenitor cells express the serotonin receptor and are susceptible to JC virus infection
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DOI：
10.1128/jvi.00406-08
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发表时间：
2008-09-01
影响因子：
5.4
通讯作者：
Atwood, W. J.
中科院分区：
文献类型：
--
作者：
Schaumburg, C.;O'Hara, B. A.;Atwood, W. J.
我们研究了人胚胎干细胞来源的少突胶质祖细胞对JC病毒感染的易感性，JC病毒是进行性多灶性白质脑病（PML）的病原体。使用人胚胎干细胞系H7来获得表达少突胶质细胞祖细胞特异性标记物NG2的富集细胞群。这些细胞表达JC病毒的5HT（2a）受体（5HT（2a）R），并且对感染高度敏感。通过抗511 T（2a）R抗体和5HT（2a）R拮抗剂ritanserin和ketanserin治疗，感染减少。这是首次证明人胚胎干细胞来源的少突胶质细胞祖细胞对JC病毒感染敏感，并表明准备补充PML病变中成熟少突胶质细胞的细胞也可能是病毒感染的靶点。
We studied the susceptibility of human embryonic stem cell-derived oligodendrocyte progenitor cells to infection with JC virus, the causative agent of progressive multifocal leukoencephalopathy (PML). A human embryonic stem cell line, H7, was used to derive an enriched population of cells expressing the oligodendrocyte progenitor cell-specific marker NG2. These cells expressed the 5HT(2a) receptor (5HT(2a)R) for JC virus and were highly susceptible to infection. Infection was reduced by treatment with anti-511T(2a)R antibodies and by the 5HT(2a)R antagonists ritanserin and ketanserin. This is the first demonstration that human embryonic stem cell-derived oligodendrocyte progenitor cells are susceptible to JC virus infection and indicates that cells poised to replenish mature oligodendrocytes in PML lesions may also be a target of viral infection.