Effect of hydroxyorganoboranes on synthesis, transport and N-linked glycosylation of plasma proteins.
Effect of hydroxyorganoboranes on synthesis, transport and N-linked glycosylation of plasma proteins.
复制标题
羟基有机硼烷对血浆蛋白合成、转运和 N-连接糖基化的影响。
DOI：
10.1016/0006-291x(87)91138-7
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Gallop,PM
中科院分区：
文献类型：
--
作者：
Goldberger,G;Paz,MA;Torrelio,BM;Okamoto,Y;Gallop,PM
利用最近开发的将水不溶性羟基有机硼烷化合物转移到细胞中的方法（Boradeption），我们观察到其中三种化合物对人肝癌 HepG2 细胞中蛋白质合成的抑制作用以及其中四种化合物对血浆蛋白分泌的抑制作用。这些效果是特定的，因为细胞活力不受影响并且没有观察到蛋白质分解代谢的增加。由于 N-连接碳水化合物部分的潜在变化，三种化合物导致分泌糖蛋白的电泳迁移率发生化合物特异性改变。所呈现的结果表明细胞探针的潜在新来源。
Utilizing a recently developed method (Boradeption) for transfering water-insoluble hydroxyorganoborane compounds into the cells, we observed inhibition of protein synthesis by three of these compounds and inhibition of secretion of plasma proteins by four of them in human hepatoma HepG2 cells. These effects were specific in that the cell viability was not affected and an increase in protein catabolism was not observed. Three compounds caused a compound-specific alterations in the electrophoretic mobility of secreted glycoproteins due to underlying changes in the N-linked carbohydrate moieties. Results presented suggest a potential new source of cellular probes.