Extracts and components of Ficus carica leaves suppress survival, cell cycle, and migration of triple-negative breast cancer MDA-MB-231 cells.
Extracts and components of Ficus carica leaves suppress survival, cell cycle, and migration of triple-negative breast cancer MDA-MB-231 cells.
复制标题
无花果叶的提取物和成分可抑制三阴性乳腺癌 MDA-MB-231 细胞的存活、细胞周期和迁移
DOI：
10.2147/ott.s171601
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发表时间：
2018
影响因子：
4
通讯作者：
Hu X
中科院分区：
文献类型：
--
作者：
Zhang Y;Wan Y;Huo B;Li B;Jin Y;Hu X
无花果的产物已被用于传统医学中以治疗许多疾病。本研究旨在分析F.在三阴性乳腺癌细胞系MDA-MB-231上，材料与方法采用人乳腺癌细胞株MDA-MB-231，观察F.番木瓜提取物。结果表明，F.采用MTT法测定番木瓜对细胞活力的影响。使用细胞周期分析检查细胞周期分布。使用伤口愈合测定来评价MDA-MB-231的迁移。采用定量逆转录聚合酶链反应检测Bax、p53、p21、GATA 3、ELF 5、细胞周期蛋白依赖性激酶、MMP 2和金属蛋白酶组织抑制剂的水平。结果通过对细胞生长抑制作用机制的研究，发现促凋亡基因的表达增加。此外，经处理的细胞示出了在S期增加的部分和改变的细胞周期蛋白依赖性激酶的表达，表明细胞周期停滞在S期。此外，经处理的细胞显示出降低的细胞移动性，这是转移所必需的。F.洋地黄叶、佛手柑内酯和补骨脂素具有与F.表明这两种成分可能在无花果叶提取物的抗肿瘤作用中起重要作用。番木瓜叶。结论F.番木瓜叶可能是开发抑制癌细胞生长和迁移以治疗三阴性乳腺癌的药物的良好来源。
Background Products from Ficus carica have been used in traditional medicine to treat many diseases. This study aimed to analyze anticancer effects of extracts of F. carica leaves on the triple-negative breast cancer cell line MDA-MB-231. Materials and methods The human breast cancer cell line MDA-MB-231 was used to evaluate effects of F. carica extracts. Effects of F. carica on cell viability were evaluated using MTT assays. Cell-cycle distribution was examined using cell-cycle analysis. Wound-healing assays were used to evaluate migration of MDA-MB-231. Quantitative reverse-transcription polymerase chain reaction was used to detect levels of Bax, p53, p21, GATA3, ELF5, cyclin-dependent kinases, MMP2, and tissue inhibitors of metalloproteinase. Results We investigated the mechanism of anti-growth effects, and found that the expressions of genes that promote apoptosis were increased. In addition, the treated cells illustrated increased portion at S phase and changed expression of cyclin-dependent kinases, demonstrating cell-cycle arrest at the S phase. Furthermore, treated cells showed decreased cell mobility, which is essential for metastasis. Two of the active components of F. carica leaves, bergapten and psoralen, had similar anticancer effects as F. carica leaf extracts, indicating that these two components might play important roles in anticancer effects of F. carica leaves. Conclusion Our findings suggest that F. carica leaves might be a good source to develop drugs for suppressing cancer-cell growth and migration to treat triple-negative breast cancers.