Characterization of microbial consortia in a terephthalate-degrading anaerobic granular sludge system.
Characterization of microbial consortia in a terephthalate-degrading anaerobic granular sludge system.
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DOI：
10.1099/00221287-147-2-373
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发表时间：
2001-02
期刊：
影响因子：
1.5
通讯作者：
Jer Horng Wu;Wen Tso Liu;I. Tseng;Sheng-Shung Cheng
中科院分区：
文献类型：
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作者：
Jer Horng Wu;Wen Tso Liu;I. Tseng;Sheng-Shung Cheng
利用分子生物学技术对厌氧颗粒污泥系统中微生物的组成和空间分布进行了研究。16 SrDNA克隆文库和序列分析表明，106个细菌克隆中78.5%属于变形菌纲的delta亚类，其余的克隆分别归属于绿色非硫细菌（7.5%）、增效菌（0.9%）和未鉴定菌（13.1%）。大多数的细菌克隆在δ-变形菌门形成了一个新的组不包含已知的细菌分离株。在72个古菌克隆中，甲烷菌属和甲烷菌属的比例分别为81.7%和18.3%。颗粒内的微生物种群的空间定位确定通过透射电子显微镜和荧光原位杂交与寡核苷酸探针靶向新的三角洲-变形菌群，acetoclastic Methanosaeta，和hydrogenotrophic Methanoacellum和成员的甲烷杆菌科。新的组包括至少两个不同的群体具有相同的杆状形态，这构成了超过87%的细菌细胞总数，并与产甲烷种群密切相关，形成一个非分层的颗粒结构。这一新的群体被认为是主要的细菌种群参与对苯二甲酸降解产甲烷颗粒财团。
The microbial composition and spatial distribution in a terephthalate-degrading anaerobic granular sludge system were characterized using molecular techniques. 16S rDNA clone library and sequence analysis revealed that 78.5% of 106 bacterial clones belonged to the delta subclass of the class Proteobacteria; the remaining clones were assigned to the green non-sulfur bacteria (7.5%), Synergistes (0.9%) and unidentified divisions (13.1%). Most of the bacterial clones in the delta-Proteobacteria formed a novel group containing no known bacterial isolates. For the domain Archaea, 81.7% and 18.3% of 72 archaeal clones were affiliated with Methanosaeta and Methanospirillum, respectively. Spatial localization of microbial populations inside granules was determined by transmission electron microscopy and fluorescent in situ hybridization with oligonucleotide probes targeting the novel delta-proteobacterial group, the acetoclastic Methanosaeta, and the hydrogenotrophic Methanospirillum and members of Methanobacteriaceae. The novel group included at least two different populations with identical rod-shape morphology, which made up more than 87% of the total bacterial cells, and were closely associated with methanogenic populations to form a nonlayered granular structure. This novel group was presumed to be the primary bacterial population involved in the terephthalate degradation in the methanogenic granular consortium.