Polymer-Mediated Extraction for Microplate Reader Spectrophotometric Determination of ppb-Level Copper(II) Ion in Water
Polymer-Mediated Extraction for Microplate Reader Spectrophotometric Determination of ppb-Level Copper(II) Ion in Water
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用于酶标仪分光光度法测定水中 ppb 级铜 (II) 离子的聚合物介导萃取
DOI：
10.2116/analsci.14.929
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发表时间：
1998
影响因子：
1.6
通讯作者：
C. Matsubara
中科院分区：
文献类型：
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作者：
T. Saitoh;M. Haga;T. Sakurai;T. Kaise;C. Matsubara
室温下，在水溶性聚合物聚(N-异丙基丙烯酰胺)(PNIPAAm)的水溶液中，形成带负电荷的双(偏铜丙二磺酸)-铜(I)络合物。当溶液升温至40℃时，PNIPAAm变为不溶于水。然后形成体积很小的胶状聚集体(聚合物相)。在没有反离子存在的情况下，螯合物很难被萃取到聚合物相，但当有适量的季铵离子作为反离子存在时，螯合物被有效地结合在一起。络合物的回收率随着反离子浓度的增加或疏水性的增加而增加，表明带电的络合物被离子对萃取到聚合物相中。在1 mM十二烷基三甲基氯化铵存在下，络合物的回收率是定量的。在96孔微孔板上用正丙醇溶解聚合物相后，测定了铜(I)络合物在450 nm处的吸光度。铜(II)浓度在5×10~(-9)~5×10~(-7)M范围内线性关系良好，5×10~(-8)M的标准偏差为6%(n=5)。
A negatively charged metal chelate, bis(bathocuproinedisulfonic acid)-copper(I), was formed and stably solubilized in an aqueous solution of a water-soluble polymer, poly( N -isopropylacrylamide) (PNIPAAm), at room temperature. When the solution was warmed at 40°C, PNIPAAm became water-insoluble. It then formed a gum-like aggregate (polymer phase) having a very small volume. The chelate was difficult to be extracted to the polymer phase in the absence of counter ions, but was efficiently incorporated when appropriate amounts of quaternary ammonium ion are present as the counter ions. The recovery of the chelate increased with increasing the concentration or the hydrophobic properties of the counter ions, indicating the ion-pair extraction of the charged chelate into the polymer phase. In the presence of 1 mM dodecyltrimethylammonium chloride, the recovery of the chelate was quantitative. After the polymer phase was dissolved by 1-propanol in the hole of a 96-hole microplate, the absorbance at 450 nm of copper(I) chelate was measured. A slightly curved calibration was obtained in the copper(II) concentration range from 5×10^-9 M to 5×10^-7 M. The standard deviation was 6% ( n =5) for 5×10^-8 M copper(II) ions.
使用 HPLC/光热转换外差干涉法对硝基多环芳烃进行高灵敏度分析
DOI：
--
发表时间：
2019
期刊：
影响因子：
--
作者：
礒田 美紀;安倍 聡彦;原田 明
通讯作者：
原田 明