A multiplex PCR test to identify four common cattle-adapted Cryptosporidium species
A multiplex PCR test to identify four common cattle-adapted Cryptosporidium species
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DOI：
10.1017/pao.2016.2
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发表时间：
2016-04-22
期刊：
影响因子：
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通讯作者：
Katzer, Frank
中科院分区：
文献类型：
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作者：
Thomson, Sarah;Innes, Elisabeth A.;Katzer, Frank
隐孢子虫病是世界范围内引起牛新生儿肠炎的一种常见病原。牛通常感染四种不同的隐孢子虫，但其中只有一种隐孢子虫与临床疾病有关。在犊牛腹泻的情况下，物种鉴定可以表明隐孢子虫是否是病原体。此外，C.小隐孢子虫是一种人畜共患物种，因此对人类健康有影响，因此鉴定感染牛的隐孢子虫物种是重要的，特别是当农场涉及人类隐孢子虫病的爆发时。本文采用多重PCR技术，对隐孢子虫的四种常见的牛适应性隐孢子虫进行了鉴定。parvum，已经开发。该测试可以快速准确地检测牛粪便样本中的隐孢子虫物种，包括混合感染，这可能会被更常见的相同基因测序方法所遗漏。
Cryptosporidium is a well-known cause of neonatal enteritis in cattle worldwide. Cattle are commonly infected with four different species of Cryptosporidium but only one of these, Cryptosporidium parvum, is associated with clinical disease. Identification of species in cases of calf scour can give an indication if Cryptosporidium is the causative agent or not. In addition, C. parvum is a zoonotic species and so has implications for human health, for this reason it is important to identify the species of Cryptosporidium infecting cattle particularly where a farm is implicated in an outbreak of cryptosporidiosis in humans. Here a multiplex PCR test, which can identify the four common cattle-adapted Cryptosporidium species, including C. parvum, has been developed. This test allows quick and accurate detection of Cryptosporidium species in cattle fecal samples including mixed infections, which could be missed by the more common method of sequencing the same gene.