RADIATION-INDUCED COGNITIVE DYSFUNCTION - AN EXPERIMENTAL-MODEL IN THE OLD RAT
RADIATION-INDUCED COGNITIVE DYSFUNCTION - AN EXPERIMENTAL-MODEL IN THE OLD RAT
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DOI：
10.1016/0360-3016(94)00332-f
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发表时间：
1995-01-01
影响因子：
7
通讯作者：
DELATTRE, JY
中科院分区：
文献类型：
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作者：
LAMPROGLOU, I;CHEN, QM;DELATTRE, JY
目的：建立放射性行为障碍动物模型。方法与材料：对26只16~27个月龄Wistar大鼠进行全脑放射治疗(30Gy10次/12d)，并设对照组26只。进行了连续的行为研究，包括单向回避、双向回避和标准的操作条件反射方法(按下杠杆回避)。结果：放射治疗前，两组大鼠在水迷宫中的表现相似。放疗后1个月和3个月的疗效差异无统计学意义。在放射治疗后6-7个月，受照大鼠的单向回避百分率(23%比55%，p<等于0.001)和双向回避百分率(18%比40%，p<等于0.01)明显低于对照组。照射后7个月，与对照组相比，照射组大鼠的反应时延长(避免按压杆11.20vs.8.43 S，p<0.05)，正确反应百分率降低(水迷宫53%vs.82%)。病理检查未在光镜水平显示照射后大鼠大脑的异常。结论：常规放射治疗可导致老年大鼠行为功能障碍，主要影响记忆。该模型可用于研究辐射所致认知改变的发病机制。
Purpose: To develop a model of radiation-induced behavioral dysfunction.Methods and Materials: A course of whole brain radiation therapy (30 Gy/10 fractions/12 days) was administered to 26 Wistar rats ages 16-27 months, while 26 control rats received sham irradiation. Sequential behavioral studies including one-way avoidance, two- way avoidance, and a standard operant conditioning method (press-lever avoidance) were undertaken. In addition, rats were studied in a water maze 7 months postradiation therapy.Results: Prior to radiation therapy, both groups were similar. No difference was found 1 and 3 months postradiation therapy. At 6-7 months postradiation therapy, irradiated rats had a much lower percentage of avoidance than controls for one-way avoidance (23% vs. 55%, p less than or equal to 0.001) and two-way avoidance (18% vs. 40%, p less than or equal to 0.01). Seven months postradiation therapy the reaction time was increased (press-lever avoidance, 11.20 s vs. 8.43 s, p less than or equal to 0.05) and the percentage of correct response was lower (water maze, 53% vs. 82%) in irradiated rats compared with controls. Pathological examination did not demonstrate abnormalities of the irradiated brains at the light microscopic level.Conclusion: Behavioral dysfunction affecting mainly memory can be demonstrated following conventional radiation therapy in old rats. This model can be used to study the pathogenesis of radiation-induced cognitive changes.