PHYSICAL MAPPING OF THE 5S RIBOSOMAL-RNA GENES ON RICE CHROMOSOME-11
PHYSICAL MAPPING OF THE 5S RIBOSOMAL-RNA GENES ON RICE CHROMOSOME-11
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DOI：
10.1007/bf00283259
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发表时间：
1994-10-28
期刊：
影响因子：
--
通讯作者：
FUKUI, K
中科院分区：
文献类型：
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作者：
KAMISUGI, Y;NAKAYAMA, S;FUKUI, K
利用原位杂交技术将一个5SrDNA基因定位于粳稻第11染色体上。采用直接克隆和直接标记的方法制备生物素标记的DNA探针，并通过成像方法将该位点定位于11号染色体短臂（11p1.1）的近端区域。信号位点与着丝粒之间的距离为4.0个任意单位，其中短臂的总长度为43.3个单位。在水稻中物理鉴定和定位的5SrDNA位点被命名为5SRrn。这里报道的5S rDNA位点的位置不同于在籼稻，这种差异的可能原因进行了讨论。5S rDNA的DNA序列也有报道。
One 5S ribosomal RNA gene (5S rDNA) locus was localized on chromosome 11 of japonica rice by in situ hybridization. The biotinylated DNA probe used was prepared by direct cloning and direct labeling methods, and the locus was localized to the proximal region of the short arm of chromosome 11 (11p1.1) by imaging methods. The distance between the signal site and the centromere is 4.0 arbitrary units, where the total length of the short arm is 43.3 units. The 5S rDNA locus physically identified and mapped in rice was designated as 5SRrn. The position of the 5S rDNA locus reported here differs from that in indica rice; possible reasons for this difference are discussed. DNA sequences of 5S rDNA are also reported.