A bacteriophage JN02 infecting multidrug-resistant Shiga toxin-producing Escherichia coli: isolation, characterisation and application as a biocontrol agent in foods
A bacteriophage JN02 infecting multidrug-resistant Shiga toxin-producing Escherichia coli: isolation, characterisation and application as a biocontrol agent in foods
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感染多重耐药产志贺毒素大肠杆菌的噬菌体 JN02：分离、表征及其作为食品生物防治剂的应用
DOI：
10.1111/ijfs.15070
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发表时间：
2021
影响因子：
3.3
通讯作者：
Wu Guo-Ping
中科院分区：
文献类型：
--
作者：
Li Ya-Ke;Wu Xin;Chen Hu;Zhao Yuan-Yang;Shu Mei;Zhong Chan;Wu Guo-Ping
噬菌体作为一种新型的耐多药滋贺毒素大肠杆菌（STEC）生物防治剂，正日益受到人们的重视。在这项研究中，一个有毒的噬菌体JN 02能够感染MDR STEC的分离，其特征在于和评估其在减少MDR STEC在食品中的功效。一步生长和稳定性试验表明，JN 02具有相对较短的潜伏期（15 min）、较大的爆发大小（69 PFU/cell）和较高的理化稳定性（温度（30-60°C）、pH（2-11）、0.1%、1%猪胆汁盐、杀生物剂和食品添加剂（100%氯仿、0.1%乙酸、1%山梨酸钾）。在现场测试中，18 E.大肠杆菌对JN 02敏感。在电镀效率（EOP）中，4 E.大肠杆菌对JN 02敏感。电镜和基因组测序结果表明，JN 02属于肌尾病毒科（Myoviridae）。重要的是，在JN 02的基因组中未鉴定出已知的毒力相关基因、溶原基因和抗生素基因。当在4 °C下用JN 02处理时，在牛奶（无活细胞计数）和牛肉样品（减少1.0-1.9 log 10 CFU cm− 2）中MDR STEC的活细胞计数显著（P <0.05）减少。结果表明，JN 02有可能成为一种新型的天然生物防治剂。
Bacteriophages are increasingly considered as novel biocontrol agents against Multidrug-resistant (MDR) Shiga toxin-producingEscherichia coli(STEC). In this study, a virulent phage JN02 capable of infecting MDR STEC was isolated, characterised and evaluating its efficacy in reducing MDR STEC in foods. One-step growth and stability assay showed that JN02 had a relatively short latent period (15 min), large burst sizes (69 PFU/cell), and high physicochemical stability (temperature (30–60°C), pH (2–11), 0.1%, 1% pig bile salt, biocides and food additives (100% chloroform, 0.1% acetic acid, 1% potassium sorbate). In spot tests, 18E. colistrains were sensitive to JN02. In efficiency of plating (EOP), 4E. colistrains were sensitive to JN02. Electron microscopy and genome sequencing showed that JN02 belongs to the familyMyoviridae. Importantly, no known virulence-associated, lysogenic and antibiotic genes were identified in the genome of JN02. Viable cell counts of MDR STEC were significantly (P <0.05) reduced in milk (no viable counts) and beef samples (1.0–1.9 log10CFU cm−2reduction) when treated with JN02 at 4 °C. The results indicated that JN02 might be a new potential natural biological control agent for MDR STEC in foods.