Effect of substitution of photo-cross-linker in photochemical cytosine to uracil transition in DNA.
Effect of substitution of photo-cross-linker in photochemical cytosine to uracil transition in DNA.
复制标题
DNA 中光化学胞嘧啶到尿嘧啶转变中光交联剂取代的影响。
DOI：
10.1016/j.bmcl.2017.06.042
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发表时间：
2017
影响因子：
2.7
通讯作者：
K. Fujimoto
中科院分区：
文献类型：
--
作者：
Siddhant Sethi;Yasuharu Takashima;Shigetaka Nakamura;K. Fujimoto
基因组编辑是蛋白质工程、遗传疾病治疗和非天然蛋白质生产的重要技术。目前用于基因组编辑的酶和化学方法的缺点是它们对体内研究的适用性有限。此外，非酶促方法，如使用3-氰基乙烯基咔唑（CNVK）的光化学DNA编辑，需要高温来影响胞嘧啶到尿嘧啶的转化。为了克服这一局限性，我们开发了基于CNVK、3-甲氧羰基咔唑、3-羧基乙烯基咔唑和3-羰基酰胺基乙烯基咔唑的新型光交联剂。使用3-羧基乙烯基咔唑导致更大的加速脱氨基反应比实现与CNVK。影响超快光响应核苷加速脱氨基反应能力的最可能因素是含有光交联剂的寡脱氧核苷酸的极性和亲水性。
Genome editing is an important technique for protein engineering, treatment of genetic disorders, and production of non-native proteins. A shortcoming of current enzymatic and chemical methods for genome editing is their limited applicability forin vivostudies. In addition, non-enzymatic methods, such as photochemical DNA editing using 3-cyanovinylcarbazole (CNVK), require high temperatures to affect cytosine to uracil transformations. To overcome this limitation, we developed new photo-cross-linkers based onCNVK, 3-methoxycarbonlycarbazole, 3-carboxyvinylcarbazole, and 3-carbonylamidevinylcarbazole. The use of 3-carboxyvinylcarbazole resulted in greater acceleration of the deamination reaction than that achieved withCNVK. The most likely factors affecting the ability of ultrafast photo-responsive nucleosides to accelerate the deamination reaction are polarity and hydrophilicity of the oligodeoxyribonucleotides that contain photo-cross-linker.
影响因子：
15
作者：
Sklenak, S;Yao, LS;Yan, HG
通讯作者：
Yan, HG
DOI：
10.1021/jp044828
发表时间：
2005-03
期刊：
The journal of physical chemistry. B
影响因子：
--
作者：
L. Yao;S. Sklenak;Honggao Yan;R. Cukier
通讯作者：
L. Yao;S. Sklenak;Honggao Yan;R. Cukier