Proteome analysis of Arabidopsis thaliana by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry
Proteome analysis of Arabidopsis thaliana by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry
复制标题
DOI：
10.1002/pmic.200401062
复制
发表时间：
2005-05-01
期刊：
影响因子：
3.4
通讯作者：
Gobom, J
中科院分区：
文献类型：
--
作者：
Giavalisco, P;Nordhoff, E;Gobom, J
在本研究中，我们展示了最近测序的植物拟南芥的大规模蛋白质组分析结果。根据先前发布的连续蛋白质提取方案，我们从八种不同的拟南芥组织（初生叶、叶、茎、长角果、幼苗、种子、根和花序）中制备了蛋白质提取物，并通过二维凝胶电泳对其进行了分析。从其中三个组织（即初生叶、长角果和幼苗）总共切下 6000 个蛋白质点，并通过基质辅助激光解吸/电离飞行时间质谱肽质量指纹分析所含蛋白质。这导致了 2943 个点中包含的蛋白质的鉴定，这些蛋白质被发现是 663 个不同基因的产物。在本报告中，我们介绍并讨论了植物蛋白质组分析的方法学和生物学结果。
in the present study we show results of a large-scale proteome analysis of the recently sequenced plant Arabidopsis thaliana. On the basis of a previously published sequential protein extraction protocol, we prepared protein extracts from eight different A. thaliana tissues (primary leaf, leaf, stem, silique, seedling, seed, root, and inflorescence) and analysed these by two-dimensional gel electrophoresis. A total of 6000 protein spots, from three of these tissues, namely primary leaf, silique and seedling, were excised and the contained proteins were analysed by matrix assisted laser desorption/ionisation time of flight mass spectrometry peptide mass fingerprinting. This resulted in the identification of the proteins contained in 2943 spots, which were found to be products of 663 different genes. In this report we present and discuss the methodological and biological results of our plant proteome analysis.