Conductive Silk-Polypyrrole Composite Scaffolds with Bioinspired Nanotopographic Cues for Cardiac Tissue Engineering.
Conductive Silk-Polypyrrole Composite Scaffolds with Bioinspired Nanotopographic Cues for Cardiac Tissue Engineering.
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DOI：
10.1039/c8tb01116h
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发表时间：
2018-11
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影响因子：
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通讯作者：
Jonathan H. Tsui;Nicholas Ostrovsky-Snider;D. Yama;Jordan D. Donohue;J. Choi;Rakchanok Chavanachat;
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文献类型：
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作者：
Jonathan H. Tsui;Nicholas Ostrovsky-Snider;D. Yama;Jordan D. Donohue;J. Choi;Rakchanok Chavanachat;
我们报道了由导电酸修饰的丝素-聚吡啶（AMSF+PPy）底物制成的仿生心脏支架的开发，该支架具有纳米级的脊和凹槽，使人想起天然心肌细胞外基质（ECM）的地形，以增强培养的人类多能干细胞（hPSC）来源的心肌细胞的结构和功能特性。在整个制造和功能化过程中都保持了纳米模式的保真度，并且由于纳米形貌特征的存在而不会导致导电行为的损失。AMSF+PPy底物具有生物相容性和稳定性，在21天的培养期间保持较高的细胞活力，同时没有出现PPy分层的迹象。各向异性地形线索的存在导致细胞组织和肌节发育的增加，而导电线索促进了连接蛋白43 （Cx43）的表达和极化的显著改善，Cx43是细胞-细胞电偶联的关键调节因子。仿生地形和电导率的结合也增加了编码参与调节成熟人类心脏组织收缩和电生理功能的关键蛋白的基因的表达。
We report on the development of bioinspired cardiac scaffolds made from electroconductive acid-modified silk fibroin-poly(pyrrole) (AMSF+PPy) substrates patterned with nanoscale ridges and grooves reminiscent of native myocardial extracellular matrix (ECM) topography to enhance the structural and functional properties of cultured human pluripotent stem cells (hPSC)-derived cardiomyocytes. Nanopattern fidelity was maintained throughout the fabrication and functionalization processes, and no loss in conductive behavior occurred due to the presence of the nanotopographical features. AMSF+PPy substrates were biocompatible and stable, maintaining high cell viability over a 21-day culture period while displaying no signs of PPy delamination. The presence of anisotropic topographical cues led to increased cellular organization and sarcomere development, and electroconductive cues promoted a significant improvement in the expression and polarization of connexin 43 (Cx43), a critical regulator of cell-cell electrical coupling. The combination of biomimetic topography and electroconductivity also increased the expression of genes that encode key proteins involved in regulating the contractile and electrophysiological function of mature human cardiac tissue.