Influence of Feeding and Oviposition by Phytophagous Pentatomids on Photosynthesis of Herbaceous Plants
Influence of Feeding and Oviposition by Phytophagous Pentatomids on Photosynthesis of Herbaceous Plants
复制标题
植食性蝽取食产卵对草本植物光合作用的影响
DOI：
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发表时间：
2010
影响因子：
2.3
通讯作者：
F. Loreto
中科院分区：
文献类型：
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作者：
V. Velikova;G. Salerno;Francesca Frati;E. Peri;E. Conti;S. Colazza;F. Loreto
植食性昆虫取食可改变寄主植物的光合活性。研究了食草蝽类昆虫黑翅蝽和绿绿蝽取食和产卵对甘蓝和菜豆光合参数的影响。首先，我们测量了光合气体交换，叶绿素荧光成像，并诱导挥发性有机化合物（VOC）的排放后立即喂养，并在喂养后的一段时间。B的光合作用迅速而显著地下降。被食虫侵染的甘蓝和P.vulgaris。气孔导度并没有与光合作用成比例地降低，相反，光合作用的抑制可能是由于叶肉中CO2的扩散减少。我们还测量了产卵本身和产卵与光合参数上的喂养。甘蓝叶片中的光合作用受到M. histrionica，即使产卵与摄食活动无关。高分辨率叶绿素荧光成像显示，取食和产卵对光化学的损害仅限于受害区域。相比之下，光化学产量暂时增加，在未受影响的地区的攻击叶片，表明开始的补偿反应。挥发性有机化合物（VOC）的测量显示，喂养受损的植物没有排放可检测到的VOC量，表明细胞损伤（甲醇和绿色叶挥发物）。然而，M. histrionica诱导萨沃伊白菜叶片释放单萜和倍半萜。这两类萜类化合物诱导的不同时间过程可能反映了两种不同的生物合成途径的诱导，并表明这些萜类化合物在三营养相互作用中的不同作用。
Feeding by herbivorous insects may change photosynthetic activity of host plants. We studied how feeding and oviposition by herbivorous stink bugs, Murgantia histrionica and Nezara viridula (Heteroptera: Pentatomidae), affect photosynthetic parameters of Brassica oleracea (savoy cabbage) and Phaseolus vulgaris (French bean). First, we measured photosynthetic gas exchange, chlorophyll fluorescence imaging, and emission of induced volatile organic compounds (VOC) immediately after feeding and during a post-feeding period. Photosynthesis decreased rapidly and substantially in B. oleracea and P. vulgaris infested by feeding bugs. Stomatal conductance did not decrease proportionally with photosynthesis; instead, inhibition of photosynthesis likely was due to a reduced diffusion of CO2 in the mesophyll. We also measured the impact of oviposition per se and oviposition associated with feeding on photosynthetic parameters. A surprisingly large inhibition of photosynthesis was detected in cabbage leaves in response to oviposition by M. histrionica, even when oviposition was not associated with feeding activity. High resolution chlorophyll fluorescence imaging revealed that the damage to photochemistry caused by feeding and oviposition was restricted to the attacked areas. By contrast, the photochemical yield increased temporarily in the unaffected areas of the attacked leaves, indicating the onset of a compensatory response. Measurement of volatile organic compounds (VOC) revealed that feeding-damaged plants did not emit detectable amounts of VOC, indicating cellular damage (methanol and green leaf volatiles). However, feeding by M. histrionica induced emission of mono- and sesquiterpenes in savoy cabbage leaves. The different time-course of the induction of these two classes of terpenes may reflect the induction of two different biosynthetic pathways and indicate different roles of these terpenoids in tritrophic interactions.