D1 AND D2 RECEPTOR GENE-EXPRESSION IN THE RAT FRONTAL-CORTEX - CELLULAR-LOCALIZATION IN DIFFERENT CLASSES OF EFFERENT NEURONS
D1 AND D2 RECEPTOR GENE-EXPRESSION IN THE RAT FRONTAL-CORTEX - CELLULAR-LOCALIZATION IN DIFFERENT CLASSES OF EFFERENT NEURONS
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DOI：
10.1111/j.1460-9568.1995.tb01092.x
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发表时间：
1995-05-01
影响因子：
3.4
通讯作者：
LEMOINE, C
中科院分区：
文献类型：
--
作者：
GASPAR, P;BLOCH, B;LEMOINE, C
额叶皮质的多巴胺能输入在运动和认知功能中具有重要作用，这些作用由D1型和D2型多巴胺受体介导，尽管所涉及的神经回路尚未完全了解。我们使用原位杂交来确定D1和D2受体mRNA在大鼠额叶皮质的细胞定位。在相同的动物中使用逆行追踪来鉴定主要的皮质传出群体，将荧光金注射到额叶皮质的不同皮质靶区中，并将切片与D1和D2 S-35标记的cRNA探针杂交，在所研究的所有皮质区域中都存在含D1和D2 mRNA的神经元，其中内侧前额叶中的表达更高，岛叶和扣带回皮质中D1 mRNA表达较低，运动皮质和顶叶皮质中D1 mRNA表达较低，Vlb层中含D1 mRNA的神经元最多;它们也存在于所有皮质层的Via和V层以及内侧前额叶、扣带回和岛叶区的II层，荧光金双标记结果表明，D1 mRNA在皮质、皮质丘脑和皮质纹状体神经元中均有表达。含D2 mRNA的神经元主要分布在皮质纹状体和皮质皮层的第V层，皮质脊髓和皮质桥脑的神经元中均未发现D1和D2 mRNA。本研究结果表明，D1和D2受体基因在传出皮质群体中表达，其中D1的表达更高。尽管在某些皮质层中存在重叠，但D1和D2受体基因的表达对不同类别的锥体神经元具有特异性。
The dopaminergic input to the frontal cortex has an important role in motor and cognitive functions, These effects are mediated by dopamine receptors both of type D1 and of type D2, although the neural circuits involved are not completely understood. We used in situ hybridization to determine the cellular localization of D1 and D2 receptor mRNAs in the rat frontal cortex. Retrograde tracing was used in the same animals to identify the main cortical efferent populations, Fluorogold was injected into the different cortical targets of the frontal cortex and sections were hybridized with D1 and D2 S-35-labelled cRNA probes, D1 and D2 mRNA-containing neurons were present in all the cortical areas investigated, with greater expression in the medial prefrontal, insular and cingulate cortexes and lower expression in the motor and parietal cortexes, Neurons containing D1 mRNA were most abundant in layer Vlb; they were also present in layers Via and V of all cortical layers and in layer II of the medial prefrontal, cingulate and insular areas, Double labelling with fluorogold demonstrated that D1 mRNA was present in corticocortical, corticothalamic and corticostriatal neurons. Neurons containing D2 mRNA were essentially restricted to layer V, but only in corticostriatal and corticocortical neurons, Neither D1 nor D2 mRNA was found in corticospinal or corticopontine neurons. The present results demonstrate that D1 and D2 receptor genes are expressed in efferent cortical populations, with higher expression for D1, In spite of an overlap in some cortical layers, the expression of D1 and D2 receptor genes is specific for different categories of pyramidal neurons.