The effect of hypotonic and hypertonic solutions on fibroblasts of the embryonic chick heart in vitro.
The effect of hypotonic and hypertonic solutions on fibroblasts of the embryonic chick heart in vitro.
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DOI：
10.1084/jem.30.6.617
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发表时间：
1919-12-01
影响因子：
15.3
通讯作者：
Hogue, MJ
中科院分区：
文献类型：
--
作者：
Hogue, MJ
方法。实验所用仪器及玻璃器皿均已消毒；在准备培养物的过程中，仪器在火焰中持续消毒，因此培养物的感染很少。将溶液加热后倒入小型培养皿中。在洛克-刘易斯溶液中无菌地从鸡胚中取出心脏，并转移到另一个含有约10份培养基的培养皿中。在这里，它被切成小块，通过移液管转移到一个盖子上：玻璃，它立即倒置在一个硬凡士林环上的凹载玻片。路易斯夫妇的这种方法蒸发的机会很小，因为纸巾可以放在盖子玻璃上，几秒钟就密封好了。当组织被植入高渗或低渗溶液时，心脏在从胚胎中取出后立即被放置在液体中；因此，组织永远不会与其他可能稀释培养基的溶液接触。
Method.All the instruments and glassware used in the experiments' were sterilized; the instruments were sterilized in a flame continually during the preparation of the cultures, so that infection of the cultures was rare. The solution was warmed and poured into small Petri dishes. The heart was aseptically removed from the chick embryo in Locke-Lewis solution and transferred to another Petri dish containing about 10 co. of the medium. Here it was cut into small pieces and transferred by means of a pipette to a cover: glass, which was immediately inverted over a hard vaseline ring on a depression slide. The chance of evaporation by this method of the Lewises is very small, as the tissue can be placed on the cover-glass and sealed in a few seconds. When the tissue was to be planted in hypertonic or hypotonic solution the heart was placed in the liquid immediately after its removal from the embryo; the tissue was therefore never in contact with other solutions that might possibly dilute the medium.