Combinatorial Fgf and Bmp signalling patterns the gastrula ectoderm into prospective neural and epidermal domains
Combinatorial Fgf and Bmp signalling patterns the gastrula ectoderm into prospective neural and epidermal domains
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DOI：
10.1242/dev.01227
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发表时间：
2004-08-01
期刊：
影响因子：
4.6
通讯作者：
Wilson, SW
中科院分区：
文献类型：
--
作者：
Kudoh, T;Concha, ML;Wilson, SW
在鱼类和两栖类动物中的研究表明，分级BMP信号传导活性调节原肠胚的背腹（DV）图案。在外胚层中，认为高水平的BMP活性促进腹侧的表皮发育，而从组织器发出的分泌的BMP拮抗剂诱导背侧的神经组织。然而，在斑马鱼胚胎中，注定要为脊髓做出贡献的细胞区域一直延伸到原肠胚的腹侧，离组织者很远。我们发现，在植物（躯干和尾巴）地区的斑马鱼原肠胚，神经规格启动在所有DV位置的外胚层的方式，是不受BMP活性水平和独立的器官来源的信号。相反，我们发现FGF活性是诱导植物性前瞻性神经标志物所必需的，并且可以在不抑制BMP活性的情况下这样做。我们进一步表明，BMP信号传导确实发生在植物的前瞻性神经域和BMP活性促进通过尾部的命运由这个组织。
Studies in fish and amphibia have shown that graded Bmp signalling activity regulates dorsal-to-ventral (DV) patterning of the gastrula embryo. In the ectoderm, it is thought that high levels of Bmp activity promote epidermal development ventrally, whereas secreted Bmp antagonists emanating from the organiser induce neural tissue dorsally. However, in zebrafish embryos, the domain of cells destined to contribute to the spinal cord extends all the way to the ventral side of the gastrula, a long way from the organiser. We show that in vegetal (trunk and tail) regions of the zebrafish gastrula, neural specification is initiated at all DV positions of the ectoderm in a manner that is unaffected by levels of Bmp activity and independent of organiser-derived signals. Instead, we find that Fgf activity is required to induce vegetal prospective neural markers and can do so without suppressing Bmp activity. We further show that Bmp signalling does occur within the vegetal prospective neural domain and that Bmp activity promotes the adoption of caudal fate by this tissue.