Juvenile Hormone Epoxide Hydrolase: a Promising Target for Hemipteran Pest Management.
Juvenile Hormone Epoxide Hydrolase: a Promising Target for Hemipteran Pest Management.
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DOI：
10.1038/s41598-017-00907-0
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发表时间：
2017-04-11
影响因子：
4.6
通讯作者：
Wang G
中科院分区：
文献类型：
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作者：
Tusun A;Li M;Liang X;Yang T;Yang B;Wang G
保幼激素环氧化物水解酶（JHEH）因其在昆虫保幼激素（JH）调节中的重要作用而引起人们的极大兴趣。本研究对绿盲蝽JHEH基因（AlucJHEH）进行了氨基酸序列分析、同源性分析、同源模建和对接模拟。结果揭示了AlucJHEH对JH的保守催化机制。我们的研究还表明，AlucJHEH基因的mRNA在头，胸和腹部检测到的所有生命阶段。为了在功能上表征AlucJHEH基因，设计了三个双链RNA（dsRNA）片段以靶向序列的不同区域。注射第3代果蝇，成功地敲除了目的基因的表达，并观察到存活率显著降低，同时出现蜕皮阻滞。lucorum，并表明该基因作为未来半翅目害虫控制的一个有前途的目标。
Juvenile hormone epoxide hydrolase (JHEH) has attracted great interest because of its critical role in the regulation of juvenile hormone (JH) in insects. In this study, one JHEH gene from Apolygus lucorum (AlucJHEH) was characterized in terms of deduced amino acid sequence, phylogeny, homology modeling and docking simulation. The results reveals a conserved catalytic mechanism of AlucJHEH toward JH. Our study also demonstrates that the mRNA of AlucJHEH gene was detectable in head, thorax and abdomen from all life stages. To functionally characterize the AlucJHEH gene, three fragments of double-stranded RNAs (dsRNAs) were designed to target different regions of the sequence. Injection of 3rd nymphs with dsRNA fragments successfully knocked down the target gene expression, and a significantly decreased survival rate was observed, together with a molting block, These findings confirm the important regulatory roles of AlucJHEH in A. lucorum and indicate this gene as a promising target for future hemipterans pest control.