RACK1 mediates multiple hormone responsiveness and developmental processes in Arabidopsis
RACK1 mediates multiple hormone responsiveness and developmental processes in Arabidopsis
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DOI：
10.1093/jxb/erl035
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发表时间：
2006-08-01
影响因子：
6.9
通讯作者：
Jones, Alan M.
中科院分区：
文献类型：
--
作者：
Chen, Jin-Gui;Ullah, Hemayet;Jones, Alan M.
支架蛋白RACK1（活化C激酶1受体）是多种信号转导途径的整合点。拟南芥基因组中含有三个RACK 1同源基因，但对它们的功能知之甚少。据报道，这个基因家族的一个成员，RACK1A，以前确定为拟南芥同源的烟草arcA基因，介导激素反应，并在多个发育过程中发挥调节作用。RACK1A在拟南芥中广泛表达。RACK1A的功能缺失突变导致多个发育过程中的缺陷，包括种子萌发、叶片产生和开花。Rack1a突变体在种子萌发过程中对赤霉素和油菜素内酯的敏感性降低，在种子萌发和幼苗早期发育过程中对脱落酸的敏感性降低，在不定根和侧根形成过程中对生长素的敏感性降低。这些结果首次提供了RACK1A参与多种信号转导途径的遗传学证据。
The scaffold protein RACK1 (Receptor for Activated C Kinase 1) serves as an integrative point for diverse signal transduction pathways. The Arabidopsis genome contains three RACK1 orthologues, however, little is known about their functions. It is reported here that one member of this gene family, RACK1A, previously identified as the Arabidopsis homologue of the tobacco arcA gene, mediates hormone responses and plays a regulatory role in multiple developmental processes. RACK1A expresses ubiquitously in Arabidopsis. Loss-of-function mutations in RACK1A confer defects in multiple developmental processes including seed germination, leaf production, and flowering. rack1a mutants displayed reduced sensitivity to gibberellin and brassinosteroid in seed germination, hypersensitivity to abscisic acid in seed germination and early seedling development, and hyposensitivity to auxin in adventitious and lateral root formation. These results provide the first genetic evidence that RACK1A is involved in multiple signal transduction pathways.