Replication and expression of a recombinant Sindbis virus in mosquitoes.
Replication and expression of a recombinant Sindbis virus in mosquitoes.
复制标题
重组辛德比斯病毒在蚊子中的复制和表达。
DOI：
10.1111/j.1365-2583.1995.tb00030.x
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Beaty,BJ
中科院分区：
文献类型：
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作者：
Rayms-Keller,A;Powers,AM;Higgs,S;Olson,KE;Kamrud,KI;Carlson,JO;Beaty,BJ
将含有长曲棍球病毒小片段cDNA的重组Sindbis病毒TE/3 '2 J/ANTI-S接种于三纹伊蚊体内。在时间和空间上分析病毒复制和LAC-ANTI-S RNA表达。感染后6-9天（p.i.），头部TE/3 '2 J/ANTI‐S病毒滴度达到峰值5.0 log 10 TCID 50。在p.i.感染后6天，唾液腺含4.4 log 10 TCID 50病毒;其他器官的滴度较低。LAC-ANTI-S RNA水平和病毒滴度。SIN E1抗原在蚊的多种器官系统中均有表达，但仅在某些器官的特定细胞和组织中表达。TE/3 '2 J/ANTI-S病毒在伊蚊和库蚊的唾液腺中表现出不同的细胞嗜性。
A recombinant Sindbis virus, TE/3'2J/ANTI‐S, containing LaCrosse virus small segment cDNA in antisense orientation, was inoculated intoAedes triseriatusmosquitoes. Virus replication and LAC‐ANTI‐S RNA expression were analysed temporally and spatially. TE/3'2J/ANTI‐S virus titre peaked at 5.0 log10TCID50in heads 6–9 days post infection (p.i.) and decreased to 3.4 log10TCID50by 37 days p.i. Salivary glands contained 4.4 log10TCID50of virus 6 days p.i.; titres were lower in other organs. LAC‐ANTI‐S RNA levels paralleled virus titre. SIN E1 antigen was detected in many mosquito organ systems, but in specific cells and tissues of some organs. TE/3'2J/ANTI‐S virus exhibited different cellular tropisms in salivary glands ofAedesandCulexmosquitoes.