Growth, nitrogen fixation, and nodularin production by two Baltic sea cyanobacteria
Growth, nitrogen fixation, and nodularin production by two Baltic sea cyanobacteria
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DOI：
10.1128/aem.63.5.1647-1656.1997
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发表时间：
1997-05-01
影响因子：
4.4
通讯作者：
Kononen, K
中科院分区：
文献类型：
--
作者：
Lehtimaki, J;Moisander, P;Kononen, K
在夏末，固氮蓝藻Nodularia spumigena和Aphanizomenon flos-aquae在波罗的海形成水华。N. spumigena已经引起了几起动物中毒，但波罗的海A。pos-aquae没有已知的毒性。采用间歇培养的方法，研究了环境条件对固氮菌生物量和固氮速率的影响。spumigena和A. flos-aquae的比较，并测定了N.测量泡沫生成量。在生物量和固氮速率方面，不同的固氮菌存在一定的差异。spumigena和A.观察到水华。A.水华藻类比N.泡沫。这两个物种的生物量增加与高磷酸盐浓度和伴随的细菌和非自然高无机氮浓度下降。在促进生长的条件下，细胞和生长培养基中的节球菌素浓度以及固氮率通常最高。胞内节球藻素浓度随高温、高光照、高磷酸盐浓度的升高而升高，随低盐、高盐、高无机氮浓度的升高而降低。生长培养基中的节球菌素浓度随着孵育时间的增加而增加，表明细胞裂解时细胞内的节球菌素被释放。A. flos-aquae和N. spumigena的盐度，辐照度和温度的变化可以解释这些物种在波罗的海的不同的空间和时间分布。结果表明，有毒N.在波罗的海磷浓度高、盐度适中的地区，预计在夏末可能会出现泡沫藻水华。
In late summer nitrogen-fixing cyanobacteria Nodularia spumigena and Aphanizomenon flos-aquae form blooms in the open Baltic Sea. N. spumigena has caused several animal poisonings, but Baltic A. pos-aquae is not known to be toxic. In this laboratory study, performed with batch cultures, the influences of environmental conditions on the biomass and nitrogen fixation rate of N. spumigena and A. flos-aquae were compared and the toxin (nodularin) concentration produced by N. spumigena was measured. Several differences in the biomasses and nitrogen fixation rates of N. spumigena and A. flos-aquae were observed. A. flos-aquae preferred lower irradiances, salinities, and temperatures than N. spumigena. The biomass of both species increased with high phosphate concentrations and with accompanying bacteria and decreased with unnaturally high inorganic nitrogen concentrations. Nodularin concentrations in cells and growth media, as well as nitrogen fixation rates, were generally highest under the conditions that promoted growth. Intracellular nodularin concentrations increased with high temperature, high irradiance, and high phosphate concentration and decreased with low and high salinities and high inorganic nitrogen concentrations. Nodularin concentrations in growth media increased with incubation time, indicating that intracellular nodularin was released when cells lysed. The different responses of A. flos-aquae and N. spumigena to changes in salinity, irradiance, and temperature may explain the different spatial and temporal distribution of these species in the Baltic Sea. According to the results, toxic N. spumigena blooms may be expected in late summer in areas of the Baltic Sea with high phosphorus concentrations and moderate salinity.