Perianth bottom-specific blue color development in tulip cv. Murasakizuisho requires ferric ions
Perianth bottom-specific blue color development in tulip cv. Murasakizuisho requires ferric ions
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DOI：
10.1093/pcp/pcl060
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发表时间：
2007-02-01
影响因子：
4.9
通讯作者：
Yoshida, Kumi
中科院分区：
文献类型：
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作者：
Shoji, Kazuaki;Miki, Naoko;Yoshida, Kumi
郁金香（Tulipa generiana）的全花。Murasakizuisho是紫色的，除了底部是蓝色的。为了阐明同一花瓣中不同颜色发育的机制，我们分别制备了紫色和蓝色表皮的原生质体，用高效液相色谱法测定了黄酮类成分，用质子选择微电极测定了液泡pH，用电感耦合等离子体（ICP）法测定了元素含量。化学分析表明，紫色和蓝色原生质体中花青素和黄酮醇的组成相同；delphinidin 3- o -rutinoside(1)和主要的三种黄酮醇苷，manghaslin (2), rutin(3)和mauritianin(4)。紫色和蓝色原生质体的液泡pH值分别为5.5和5.6，差异不显著。而蓝色原生质体中Fe3+含量接近9.5 mM，是紫色原生质体的25倍。将1与两种等摩尔浓度的黄酮醇混合，λ (vismax) = 539 nm，可以得到与紫色原生质体相同的紫色溶液。此外，在1-4的混合物中加入Fe3+，得到与蓝色原生质体相同的λ (vismax) = 615 nm的蓝色溶液。我们已经确定Fe3+对郁金香的蓝色发育至关重要。
The entire flower of Tulipa gesneriana cv. Murasakizuisho is purple, except the bottom, which is blue. To elucidate the mechanism of the different color development in the same petal, we prepared protoplasts from the purple and blue epidermal regions and measured the flavonoid composition by HPLC, the vacuolar pH by a proton-selective microelectrode, and element contents by the inductively coupled plasma (ICP) method. Chemical analyses revealed that the anthocyanin and flavonol compositions in both purple and blue colored protoplasts were the same; delphinidin 3-O-rutinoside (1) and major three flavonol glycosides, manghaslin (2), rutin (3) and mauritianin (4). The vacuolar pH values of the purple and blue protoplasts were 5.5 and 5.6, respectively, without any significant difference. However, the Fe3+ content in the blue protoplast was similar to 9.5 mM, which was 25 times higher than that in the purple protoplasts. We could reproduce the purple solution by mixing 1 with two equimolar concentrations of flavonol with lambda(vismax) = 539 nm, which was identical to that of the purple protoplasts. Furthermore, addition of Fe3+ to the mixture of 1-4 gave the blue solution with lambda(vismax) = 615 nm identical to that of the blue protoplasts. We have established that Fe3+ is essential for blue color development in the tulip.