DNA repair and the accumulation of oxidatively damaged DNA are affected by fruit intake in mice.
DNA repair and the accumulation of oxidatively damaged DNA are affected by fruit intake in mice.
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小鼠的 DNA 修复和氧化损伤 DNA 的积累受到水果摄入量的影响。
DOI：
10.1093/gerona/glq157
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
Bohr,VilhelmA
中科院分区：
文献类型：
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作者：
Croteau,DeborahL;deSouza-Pinto,NadjaC;Harboe,Charlotte;Keijzers,Guido;Zhang,Yongqing;Becker,Kevin;Sheng,Shan;Bohr,VilhelmA
衰老与氧化应激和DNA损伤有关。为了实现健康的老龄化，我们必须开始了解饮食如何影响细胞过程。我们假设富含水果的饮食可能会启动一个增强DNA修复的程序，从而改善基因组的完整性。C57 Bl/6 J小鼠喂食对照饮食或含有8%桃或油桃提取物的饮食14周。检测DNA修复酶活性、DNA损伤水平和基因表达变化。我们的研究表明，各种氧化DNA损伤的修复在来自喂食富含水果的饮食的小鼠的肝脏提取物中更有效。为了支持这些发现，气相色谱-质谱分析显示，与对照组相比，喂食桃子的小鼠中甲酰胺嘧啶的水平有所下降。此外，微阵列分析显示，NTH 1在桃子喂养的小鼠中上调。总之，这些结果表明，增加水果的摄入量可能会调节DNA修复的效率，导致DNA损伤水平的改变。
AGINGis associated with elevated oxidative stress and DNA damage. To achieve healthy aging, we must begin to understand how diet affects cellular processes. We postulated that fruit-enriched diets might initiate a program of enhanced DNA repair and thereby improve genome integrity. C57Bl/6 J mice were fed for 14 weeks a control diet or a diet with 8% peach or nectarine extract. The activities of DNA repair enzymes, the level of DNA damage, and gene expression changes were measured. Our study showed that repair of various oxidative DNA lesions was more efficient in liver extracts derived from mice fed fruit-enriched diets. In support of these findings, gas chromatography–mass spectrometry analysis revealed that there was a decrease in the levels of formamidopyrimidines in peach-fed mice compared with the controls. Additionally, microarray analysis revealed that NTH1 was upregulated in peach-fed mice. Taken together, these results suggest that an increased intake of fruits might modulate the efficiency of DNA repair, resulting in altered levels of DNA damage.