Effects of prolonged starvation on plasma free fatty acid levels and fatty acid composition of myocardial total lipids in the rat.
Effects of prolonged starvation on plasma free fatty acid levels and fatty acid composition of myocardial total lipids in the rat.
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DOI：
10.1093/jn/110.12.2490
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发表时间：
1980-12
期刊：
影响因子：
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通讯作者：
S. Yaffe;Armand J. Gold;Joseph Sampugna
中科院分区：
文献类型：
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作者：
S. Yaffe;Armand J. Gold;Joseph Sampugna
实验在雄性大鼠上进行，自由进食或饥饿7天。采用气相色谱法（GLC）分析了血浆游离脂肪酸（FFA）水平和在体心脏心室组织总脂质中脂肪酸的数量和组成。此外，FFA提取率和摄取率测定在离体灌注心脏使用经典的Langendorff技术。饥饿7天后，从饥饿动物的心脏心室中获得的总脂质脂肪酸的分布和浓度与对照组有很大不同。特别是，20：5和22：6碳多不饱和脂肪酸显着升高。此外，总血浆游离脂肪酸水平升高，游离脂肪酸提取率增加，作为长期饥饿的结果。在饥饿大鼠心室中观察到的20-和22-碳多不饱和脂肪酸的蓄积可能是由于FFA的可用性和提取增加所致。
Experiments were carried out on male rat fed ad libitum or starved for a period of 7 days. Plasma levels of free fatty acids (FFA) and the quantity and composition of fatty acids in total lipids of heart ventricular tissue in vivo were analyzed by gas liquid chromatography (GLC). In addition, FFA extraction ratios and uptake rates were determined in isolated perfused hearts using the classical Langendorff technique. After 7 days of starvation, distribution and concentrations obtained for total lipid fatty acids from heart ventricles of starved animals were substantially different from those of controls. In particular, 20:5 and 22:6 carbon polyunsaturated fatty acids were significantly elevated. Also, total plasma FFA level was elevated and FFA extraction ratios were increased as a result of prolonged starvation. Accumulation of 20- and 22-carbon polyunsaturated fatty acids observed in ventricles of starved rats may have resulted from increased availability and extraction of FFA.