Autophagy in Normal and Abnormal Early Human Pregnancies
Autophagy in Normal and Abnormal Early Human Pregnancies
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DOI：
10.1177/1933719114565036
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发表时间：
2015-07-01
影响因子：
2.9
通讯作者：
Marconi, Anna Maria
中科院分区：
文献类型：
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作者：
Avagliano, Laura;Terraneo, Laura;Marconi, Anna Maria
自噬是一种诱导性分解代谢过程，细胞通过该过程降解和回收物质以在压力、饥饿和缺氧中生存。本研究的目的是评估胎儿-母体界面的自噬，评估人类妊娠早期的自噬参与情况，并探讨早期妊娠结局异常时的自噬修饰。样本采集自妊娠早期正常妊娠、合法终止妊娠和妊娠早期偶发性自然流产。通过透射电子显微镜、免疫组织化学、免疫荧光和蛋白质印迹研究了绒毛和蜕膜样品中的自噬。在细胞滋养层、合体滋养层、绒毛外滋养层和蜕膜基质细胞中发现自噬标记物。自噬在生理上参与正常妊娠早期。与正常妊娠相比，自然流产由于合体滋养层中自噬泡浓度的增加，导致绒毛样本中自噬表达增加，这表明细胞响应微环境挑战的细胞保护机制。
Autophagy is an inducible catabolic process by which cells degrade and recycle materials to survive stress, starvation, and hypoxia. The aim of this study was to evaluate autophagy at the fetal-maternal interface, to assess autophagy involvement during the early phase of human gestation, and to explore autophagic modification in case of early abnormal pregnancy outcome. Specimens were collected from first-trimester normal gestations undergoing legal termination of pregnancy and first-trimester sporadic spontaneous miscarriages. Autophagy was studied in villous and decidual samples by transmission electron microscopy, immunohistochemistry, immunofluorescence, and Western blotting. Autophagy markers were found in cytotrophoblast, syncytiotrophoblast, extravillous trophoblast, and decidual stromal cells. Autophagy is physiologically involved in early normal gestation. Compared with normal pregnancy, spontaneous miscarriage presents an increase in autophagy expression in villous specimens due to an increment in concentration of autophagic vacuole in syncytiotrophoblast, suggesting a cytoprotective mechanism of the cells to respond to microenvironmental challenge.