Calcium-Dependent Flagellar Motility Activation in Chlamydomonas reinhardtii in Response to Mechanical Agitation
Calcium-Dependent Flagellar Motility Activation in Chlamydomonas reinhardtii in Response to Mechanical Agitation
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DOI：
10.1002/cm.20402
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发表时间：
2009-09-01
影响因子：
--
通讯作者：
Kamiya, Ritsu
中科院分区：
文献类型：
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作者：
Wakabayashi, Ken-ichi;Ide, Takahiro;Kamiya, Ritsu
衣原体鞭毛的跳动被发现是由机械刺激激活。在野生型细胞悬浮液涡旋后，细胞的平均游动速度立即从130 μ m/秒增加到150 μ m/秒，这是由于鞭毛拍频从近似60 Hz升高到近似70 Hz，而鞭毛波形没有可检测的变化。这种反应需要外臂动力蛋白。EGTA、Ca（2+）通道阻滞剂或机械敏感性通道阻滞剂可抑制此作用。在去膜和再活化细胞模型中，Ca（2+）浓度的适度增加可提高轴丝搏动频率。这些数据表明，机械搅拌增加拍频，因为它导致Ca（2+）流入鞭毛，然后激活外臂动力蛋白。细胞动力。Cytoskeleton 66：736-742，2009. (C)2009 Wiley-Liss，Inc.
Flagellar beating in Chlamydomonas was found to be activated by mechanical stimulation. Immediately after a wild-type cell suspension was vortexed, the average swimming velocity of cells increased from 130 mu m/second to 150 mu m/second, due to an elevation of flagellar beat frequency from similar to 60 Hz to similar to 70 Hz without detectable change in the flagellar waveforms. This response required outer arm dynein. Treatment with EGTA, Ca(2+)-channel blockers, or mechanosensitive-channel blockers inhibited it. In demembranated and reactivated cell models, a modest increase in Ca(2+) concentration elevated the axonemal beat frequency. These data indicate that the mechanical agitation increases beat frequency because it causes Ca(2+) influx into flagella, which then activates outer arm dynein. Cell Motil. Cytoskeleton 66: 736-742, 2009. (C) 2009 Wiley-Liss, Inc.