Applications of single-cell technology on bacterial analysis
Applications of single-cell technology on bacterial analysis
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DOI：
10.1007/s40484-019-0177-6
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发表时间：
2019-09
影响因子：
3.1
通讯作者：
Zhixin Ma;Pan Chu;Yingtong Su;Yue Yu;Hui Wen;Xiongfei Fu;Shuqiang Huang
中科院分区：
文献类型：
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作者：
Zhixin Ma;Pan Chu;Yingtong Su;Yue Yu;Hui Wen;Xiongfei Fu;Shuqiang Huang
传统上，科学家们通过批量培养的方式来研究微生物学，通过平均所有个体来得出动态或输出。然而，随着研究的深入，个体间的异质性已被证明对种群的动态和命运至关重要。结果由于技术的限制，单细胞分析方法并未广泛应用于破译单个细胞与群体之间的内在联系。自本世纪初以来，微流体、荧光标记、下一代测序和高分辨率显微镜的快速发展加速了单细胞技术的发展，并进一步促进了这些技术在细菌分析中的应用。本文从细胞内特征、细胞生理动力学和群体行为等方面综述了近年来单细胞技术在细菌分析中的应用进展，并探讨了单细胞技术在未来细菌研究中的应用前景。
BackgroundTraditionally, scientists studied microbiology through the manner of batch cultures, to conclude the dynamics or outputs by averaging all individuals. However, as the researches go further, the heterogeneities among the individuals have been proven to be crucial for the population dynamics and fates.ResultsDue to the limit of technology, single-cell analysis methods were not widely used to decipher the inherent connections between individual cells and populations. Since the early decades of this century, the rapid development of microfluidics, fluorescent labelling, next-generation sequencing, and high-resolution microscopy have speeded up the development of single-cell technologies and further facilitated the applications of these technologies on bacterial analysis.ConclusionsIn this review, we summarized the recent processes of single-cell technologies applied in bacterial analysis in terms of intracellular characteristics, cell physiology dynamics, and group behaviors, and discussed how single-cell technologies could be more applicable for future bacterial researches.