Hepatitis B virus core and core-related antigen quantitation in Chinese patients with chronic genotype B and C hepatitis B virus infection
Hepatitis B virus core and core-related antigen quantitation in Chinese patients with chronic genotype B and C hepatitis B virus infection
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DOI：
10.1111/j.1440-1746.2005.04087.x
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发表时间：
2005-11-01
影响因子：
4.1
通讯作者：
Kiyosawa, K
中科院分区：
文献类型：
--
作者：
Rokuhara, A;Sun, XH;Kiyosawa, K
背景和目标：建立了B型肝炎病毒（HBV）核心相关抗原（HBcrAg）和核心抗原（HBcAg）检测方法，用于测定血清HBV载量。本研究的目的是评估这些检测方法在中国慢性基因型B和C HBV感染患者中的临床实用性。方法：193例慢性肝炎B患者入选。采用化学发光酶免疫法检测血清HBcAg和HBcAg，采用敏感的聚合酶链反应法检测HBV-DNA。对HBV基因型B（HBV/B）和C（HBV/C）进行分析。计算并比较有无B e抗原（HBeAg）患者的HBcrAg/HBcAg比值。HBV/B组血清HBcAg、HBcAg浓度与HBV-DNA浓度呈显著正相关（分别为r = 0.79，P < 0.001和r = 0.77，P < 0.001）和HBV/C（分别为r = 0.87，P < 0.001和r = 0.90，P < 0.001）。阳性HBcAg的临界值约为4.5 log拷贝/mL，阳性HBcrAg结果的临界值约为3-4 log拷贝/mL。结论：HBcrAg和HBcAg检测可用于HBV/B和HBV/C的定量检测。这些检测的组合将是一个有价值的工具，用于分析HBV感染的临床状态。(C)2005年Blackwell Publishing Asia Pty Ltd.
Background and Aims: Hepatitis B virus (HBV) core-related antigen (HBcrAg) and HBV core antigen (HBcAg) assays were developed for the measurement of serum HBV load. The aim of this study was to assess the clinical utility of these assays in Chinese patients with chronic genotype B and C HBV infection.Methods: One hundred and ninety-three chronic hepatitis B patients were enrolled. Serum HBcrAg and HBcAg were measured by chemiluminescence enzyme immunoassay, and HBV-DNA was measured by using a sensitive polymerase chain reaction assay. The data were analyzed in patients with HBV genotype B (HBV/B) and genotype C (HBV/C). The HBcrAg/HBcAg ratio was calculated and compared between patients with and without hepatitis B e antigen (HBeAg).Results: The concentrations of HBcrAg and HBcAg showed significant positive correlation with the HBV-DNA concentration in both HBV/B (r = 0.79, P < 0.001, and r = 0.77, P < 0.001, respectively) and HBV/C (r = 0.87, P < 0.001, and r = 0.90, P < 0.001, respectively). The cut-off for a positive HBcAg corresponded to approximately 4.5 log copies/mL, and that for a positive HBcrAg result corresponded to 3-4 log copies/mL. The HBcrAg/HBcAg ratio was higher in patients with HBeAg than in those without HBeAg.Conclusions: The HBcrAg assay and HBcAg assay are clinically useful in viral quantitation of HBV/B and HBV/C. A combination of these assays would be a valuable tool for analyzing the clinical status of HBV infection. (C) 2005 Blackwell Publishing Asia Pty Ltd.