Small GTPases of the Rab family in the brain of Bombyx mori
Small GTPases of the Rab family in the brain of Bombyx mori
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DOI：
10.1007/s00418-010-0755-1
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发表时间：
2010-11
影响因子：
2.3
通讯作者：
T. Uno;Keisuke Hata;Susumu Hiragaki;Yuri Isoyama;L. Trang;Y. Uno;K. Kanamaru;H. Yamagata;Masahiko Nakamura;M. Takagi;M. Takeda
中科院分区：
文献类型：
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作者：
T. Uno;Keisuke Hata;Susumu Hiragaki;Yuri Isoyama;L. Trang;Y. Uno;K. Kanamaru;H. Yamagata;Masahiko Nakamura;M. Takagi;M. Takeda
Rab家族的小GTP酶是膜运输的关键调节剂。我们在家兔体内制备了抗家蚕Rab7蛋白（BRab 7）和抗家蚕Rab11蛋白的抗体。mori（BRab 11）的表达。抗体识别BRab7和BRab11蛋白，但不识别其他Rab蛋白。B.脑组织样品的免疫印迹。Mori为每个抗体显示了一条带。Rab11在大多数组织中表达，而Rab7在脑、卵巢和睾丸中表达。Rab7和Rab11在B.死亡仅限于大脑间部和前脑背外侧区的神经元。双标记实验表明，Rab7的免疫组织化学反应共定位与Rab11和部分与Rab8。Rab11和Rab8的免疫组织化学反应与与昼夜节律相关的蛋白之一PERIOD的免疫组织化学反应共定位。这些发现表明Rab7、Rab8和Rab11参与了B. moriand可能在昼夜节律的控制中起作用。
Small GTPases of the Rab family are key regulators of membrane trafficking. We produced antibodies against the Rab7 protein ofBombyx mori(BRab7) in rabbits, and against the Rab11 protein ofB. mori(BRab11) in mice. The antibodies recognized BRab7 and BRab11 proteins, but did not recognize other Rab proteins. Immunoblotting of samples from brain tissue ofB. morirevealed a single band for each antibody. Rab11 was expressed in most tissues, whereas Rab7 was expressed in the brain, ovary, and testis. Immunohistochemical reactivity of Rab7 and Rab11 in the brain ofB. moriwas restricted to neurons of the pars intercerebralis and dorsolateral protocerebrum. Double-labeling experiments demonstrated that immunohistochemical reactivity of Rab7 co-localized with that of Rab11 and partially with that of Rab8. Immunohistochemical reactivity of Rab11 and Rab8 co-localized with that of PERIOD, one of the proteins associated with circadian rhythm. These findings suggest that Rab7, Rab8, and Rab11 are involved in protein transport in the neurons of the brain ofB. moriand might play a role in the control of circadian rhythm.