Differentiation of commercial strains of Agaricus species in China with inter-simple sequence repeat marker
Differentiation of commercial strains of Agaricus species in China with inter-simple sequence repeat marker
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DOI：
10.1007/s11274-007-9647-5
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发表时间：
2008-08-01
影响因子：
4.1
通讯作者：
Luo, Xin-Chang
中科院分区：
文献类型：
--
作者：
Guan, Xue-Juan;Xu, Lu;Luo, Xin-Chang
近年来，蘑菇产量在世界范围内有了很大的增长。然而，对一些商业菌株的分类没有达成共识。因此，迫切需要快速、可靠的蘑菇菌种鉴定方法。从20个引物中筛选出6个ISSR引物，对我国12个主要食用菌菌株进行遗传多样性分析。结果表明，共扩增出124条谱带，其中多态性谱带占80.6%，相似系数在0.69 ~ 0.98之间。在相似系数为0.75时，12个供试菌株可分为2类。3个双孢蘑菇菌株聚为一类，7个双孢蘑菇菌株与蘑菇菌株S1和S2聚为一类。将IGS扩增产物分别用限制性内切酶Hae Ⅲ、Alu Ⅰ、Rsa Ⅰ酶切，采用基因间间隔区结合限制性片段长度多态性（IGS-RFLP）技术分析供试菌株的遗传多样性，结果表明IGS-RFLP聚类结果与ISSR聚类结果基本一致。但在区分亲缘关系较近的菌株方面，ISSR比IGS-RFLP具有上级优势。因此，ISSR标记是一种区分蘑菇属菌株的有效方法。
In recent years, Agaricus mushroom production has undergone an important increase in the world. However, there is no consensus over the taxonomy of some commercial strains. So rapid and reliable methods to identify Agaricus strains are urgently needed. In this paper, six inter-simple sequence repeat (ISSR) primers were screened from 20 primers to analyse the genetic diversities of the 12 main commercial strains of Agaricus in China. The results showed that a total of 124 bands were produced, among which 80.6% was polymorphic, the similarity coefficients ranged from 0.69 to 0.98. Twelve tested strains were divided into 2 groups on the similarity coefficient of 0.75. Three Agaricus bitorquis strains were clustered in one group, and seven Agaricus bisporus strains were clustered with Agaricus strains S1 and S2 in the other group. As comparison with ISSR, intergenic spacers combined with restriction fragment length polymorphisms (IGS-RFLP) was employed to analyse genetic diversities of the tested strains by digesting IGS amplified products with restriction enzymes Hae III, Alu I, Rsa I, respectively, and IGS-RFLP cluster results were similar with those of ISSR. However, ISSR was superior to IGS-RFLP in differentiating closely related strains. It is suggested that the ISSR marker is an alternative method to differentiate Agaricus strains.