Collision-induced dissociation pathways of yeast sphingolipids and their molecular profiling in total lipid extracts: a study by quadrupole TOF and linear ion trap-orbitrap mass spectrometry
Collision-induced dissociation pathways of yeast sphingolipids and their molecular profiling in total lipid extracts: a study by quadrupole TOF and linear ion trap-orbitrap mass spectrometry
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DOI：
10.1002/jms.997
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发表时间：
2006-03-01
影响因子：
2.3
通讯作者：
Shevchenko, A
中科院分区：
文献类型：
--
作者：
Ejsing, CS;Moehring, T;Shevchenko, A
酵母酿酒酵母合成三类鞘脂：肌醇磷酸神经酰胺（IPC）、甘露糖基肌醇磷酸神经酰胺（MIPC）和甘露糖基二肌醇磷酸神经酰胺（M（IP）（2）C）。他们的分子阴离子的串联质谱的混合四极杆飞行时间（QqTOF）仪器产生的片段肌醇含头基团，这是特定的每一个脂质类。在混合线性离子阱-轨道阱（LTQ Orbitrap）质谱仪上进行MSn分析，质量准确度优于3 ppm，可鉴别出单个分子物质中酰胺连接脂肪酸和长链碱基部分的特异性碎片离子。通过选择多个前体离子扫描的类特定碎片离子的m/z，我们在QqTOF质谱仪上分析了总脂质提取物中的酵母鞘脂。因此，QqTOF和LTQ轨道阱质谱的组合本身倾向于对重要模式生物（例如真菌和植物）中的鞘脂和甘油磷脂的分子组成进行快速、全面和结构特异性分析。版权所有（c）2006约翰威利父子有限公司。
The yeast Saccharomyces cerevisiae synthesizes three classes of sphingolipids: inositolphosphoceramides (IPCs), mannosyl-inositolphosphoceramides (MIPCs), and mannosyl-diinositolphosphoceramides (M(IP)(2)C). Tandem mass spectrometry of their molecular anions on a hybrid quadrupole time-of-flight (QqTOF) instrument produced fragments of inositol-containing head groups, which were specific for each lipid class. MSn analysis performed on a hybrid linear ion trap-orbitrap (LTQ Orbitrap) mass spectrometer with better than 3 ppm mass accuracy identified fragment ions specific for the amide-linked fatty acid and the long chain base moieties in individual molecular species. By selecting m/z of class-specific fragment ions for multiple precursor ion scanning, we profiled yeast sphingolipids in total lipid extracts on a QqTOF mass spectrometer. Thus, a combination of QqTOF and LTQ Orbitrap mass spectrometry tends itself to rapid, comprehensive and structure-specific profiling of the molecular composition of sphingolipids and glycerophospholipids in important model organisms, such as fungi and plants. Copyright (c) 2006 John Wiley & Sons, Ltd.