OXYGEN TOLERANCE OF FRESH CLINICAL ANAEROBIC BACTERIA
OXYGEN TOLERANCE OF FRESH CLINICAL ANAEROBIC BACTERIA
复制标题
DOI：
10.1128/jcm.1.2.161-164.1975
复制
发表时间：
1975-01-01
影响因子：
9.4
通讯作者：
ROSENBLATT, JE
中科院分区：
文献类型：
--
作者：
TALLY, FP;STEWART, PR;ROSENBLATT, JE
本文对57株临床分离的厌氧菌进行了耐氧性和敏感性研究。所有的有机体耐受8小时或更长的暴露于室内空气中的氧气。在不断增加的氧气浓度的菌株的生长表明，57株不同的氧敏感性从严格的耐氧厌氧菌。氧耐受性和敏感性的比较表明，最耐受的生物体（最好的生存后，长期暴露）包括厌氧菌能够生长在只有0.4%或更低的O2（严格），以及那些能够生长在高达10%的O2。最不耐受的主要是严格的厌氧菌。将接种量从10（8）个菌落形成单位/ml降低至10（6）个菌落形成单位/ml，仅产生轻微影响。这些数据表明，在临床实验室中使用台式技术进行短暂的氧气暴露不会对临床标本中存在的厌氧菌造成损害。
The oxygen tolerance and sensitivity of 57 freshly isolated anaerobic bacteria from clinical specimens was studied. All the organisms tolerated 8 h or more of exposure to oxygen in room air. Growth of the isolates in increasing oxygen concentrations demonstrated that the 57 isolates varied in oxygen sensitivity from strict to aerotolerant anaerobes. Comparison of the oxygen tolerance and sensitivity showed that the most tolerant organisms (best survival after prolonged exposure) included anaerobes capable of growth at only 0.4% or less O2 (strict) as well as those able to grow in as much as 10% O2. The least tolerant were predominately strict anaerobes. Decrease in the inoculum size from a concentration of 10(8) to 10(6) colony-forming units per ml had only a minor effect. The data indicate that the brief oxygen exposure with bench techniques in clinical laboratories would not be deleterious to the anaerobic bacteria present in clinical specimens.