Overexpression of Activin Receptor-like Kinase 7 in Breast Cancer Cells Is Associated with Decreased Cell Growth and Adhesion
Overexpression of Activin Receptor-like Kinase 7 in Breast Cancer Cells Is Associated with Decreased Cell Growth and Adhesion
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DOI：
10.21873/anticanres.11712
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发表时间：
2017-07-01
影响因子：
2
通讯作者：
Dart, D. Alwyn
中科院分区：
文献类型：
--
作者：
Hu, Tingting;Su, Fengxi;Dart, D. Alwyn
目的：探讨激活素受体样激酶7(ALK7)在乳腺癌中的表达和功能及其与疾病预后的关系，以及对乳腺癌细胞功能的影响。患者和方法：对一组乳腺癌患者的ALK7表达与病理和临床方面的关系进行了检测。利用表达载体对ALK7进行了体外细胞鉴定。结果：总体上，乳腺癌组织中ALK7转录本的水平高于正常组织。然而，在癌症队列中，ALK7转录本水平较低与预后不良相关。ALK7低表达的患者生存时间也较短。ALK7过表达可降低乳腺癌细胞的体外增殖和黏附能力。我们发现过表达的ALK7对MCF-7细胞对化疗药物的敏感性有复杂的影响。结论：ALK7在乳腺癌中的表达降低与预后不良有关。ALK7是乳腺癌细胞黏附和增殖的负性调节因子。这表明ALK7在乳腺癌中是一种潜在的肿瘤抑制因子。
Aim: To examine the expression and function of activin receptor-like kinase 7 (ALK7) in breast cancer, its association with disease prognosis, and its impact on breast cancer cell function. Patients and Methods: A cohort of patients with breast cancer were examined for ALK7 expression in association with pathological and clinical aspects. In vitro cell assays of ALK7 were investigated using an expression plasmid. Results: Overall higher levels of ALK7 transcripts were seen in the breast cancer samples vs. normal tissue. However, within the cancer cohort, lower levels of ALK7 transcript were associated with poor prognosis. Patients with lower expression of ALK7 also had shorter survival. Overexpression of ALK7 reduced proliferation and adhesion of breast cancer cells in vitro. We found that overexpressed ALK7 had complex effects on the MCF-7 cell sensitivity to chemotherapy drugs. Conclusion: Decreased expression of ALK7 in breast cancer is correlated with poor prognosis. ALK7 is a negative regulator of adhesion and proliferation of breast cancer cells. This suggests that ALK7 is a potential tumor suppressor in breast cancer.