CNR/Pcdhα family in subplate neurons, and developing cortical connectivity
CNR/Pcdhα family in subplate neurons, and developing cortical connectivity
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DOI：
10.1097/00001756-200412030-00007
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发表时间：
2004-12-03
期刊：
影响因子：
1.7
通讯作者：
Yagi, T
中科院分区：
文献类型：
--
作者：
Morishita, H;Murata, Y;Yagi, T
钙粘素相关神经元受体(CNR)/原钙粘蛋白(PcdH)家族是多种原钙粘蛋白家族中的一员，被认为是调节神经元连接形成的多种膜相关候选成分。然而，它在神经回路形成过程中的表达还没有得到详细的研究。在这里，我们使用一个保守的序列，通过免疫组织化学和原位杂交的方法，研究了该蛋白家族在新皮质连接发育过程中的表达。这些蛋白质在发育中的丘脑皮质和皮质分离轴突中被检测到，并在这些轴突束的先驱亚板神经元中被检测到。BrdU的出生日期标记和纯合子冷藏小鼠的检查证实了该基因在板下神经元中的表达。CNR/Pcdhalpha的这种表达模式表明它参与了新皮质连接的发展
The cadherin-related neuronal receptor (CNR)/protocadherin (Pcdh) a family is one of the diverse protocadherin families identified as a candidate diversified membrane-associated component regulating the formation of neuronal connectivity. However, its expression during neural circuit formation has not been examined in detail. Here, we used a conserved sequence to study the expression of this protein family during-the development of neocortical connectivity, by immunohistochemistry and in situ hybridization. The proteins were detected in developing thalamocortical and corticofugal axons, and in subplate neurons, which pioneer these axon tracts. The expression in subplate neurons was confirmed by birth-date labeling with BrdU, and by examination in homozygous reefer mice. This pattern of CNR/Pcdhalpha expression suggests its involvement in the development of neocortical connectivity