Effect of tetramethylpyrazine on primary afferent transmission mediated by P2X3 receptor in neuropathic pain states
Effect of tetramethylpyrazine on primary afferent transmission mediated by P2X3 receptor in neuropathic pain states
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DOI：
10.1016/j.brainresbull.2008.02.026
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发表时间：
2008-09-05
影响因子：
3.8
通讯作者：
Wan, Fang
中科院分区：
文献类型：
--
作者：
Gao, Yun;Xu, Changshui;Wan, Fang
神经病理性疼痛是最难治疗的疼痛类型，P2 X（3）受体在促进外周和脊髓部位的疼痛传递中起着至关重要的作用。本研究观察了川芎嗪（TMP）对P2 X（3）受体介导的神经病理性疼痛初级传入传递的影响。采用慢性压迫性损伤（CCl）模型。雄性SD大鼠30只，随机分为生理盐水组（I）、川芎嗪组（II）、假手术组（III）、四氯化碳+川芎嗪组（IV）和CCI组（V）。测定大鼠机械缩痛阈值（MWT）和热缩痛潜伏期（TWL），免疫组化检测L4/L5背根神经节（DRG）和脊髓P2 X（3）免疫反应。V组大鼠机械缩痛阈值和热缩痛潜伏期均低于I ~ III组和IV组（p < 0.05），而L4/L5背根节和脊髓P2 X（3）受体表达高于I ~ III组（p < 0.01）和IV组（p < 0.05）。第IV组的机械缩痛阈值、热缩痛潜伏期及L4/L5背根节和脊髓P2 X（3）免疫反应与第I、II、III组比较均无显著性差异（p > 0.05）。V组（CCI）的电流幅值明显大于其他组（P < 0.01）。CCI组大鼠DRG神经元α，β-亚甲基-ATP（α，β-meATP）激活电流明显高于其他组（p < 0.01）。结果表明，川芎嗪可抑制P2 X（3）受体介导的神经病理性痛的初级传入传递。(c)2008年爱思唯尔公司All rights reserved.
Neuropathic pain is the most difficult type of pain to treat. The P2X(3) receptors play a crucial role in facilitating pain transmission at peripheral and spinal sites. The present research investigated the effects of tetramethylpyrazine (TMP) on the primary afferent transmission induced by P2X(3) receptor in neuropathic pain states. Chronic constriction injury (CCl) model was adopted. Sprague-Dawley male rats (n = 30) had been randomly divided into normal saline (sham + NS) group (I), TMP group (II), sham group (III), CCl + TMP group (IV), and CCI group (V). Mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were measured and P2X(3) immunoreactivity in L4/L5 dorsal root ganglion (DRG) and spinal cord was detected by immunohistochemistry. The mechanical withdrawal threshold and thermal withdrawal latency in group V were lower than those in groups I-III or IV (p < 0.05), while P2X(3) receptor expression of L4/L5 DRG and spinal cord in group V was higher than those in groups I-III (p < 0.01) or group IV (p < 0.05). The mechanical withdrawal threshold, thermal withdrawal latency and P2X(3) immunoreactivity of L4/L5 DRG and spinal cord in group IV showed no significant difference compared with those in groups I, II or III (p > 0.05). The amplitudes of the currents in group V (CCI) were much larger than those obtained in other groups after application of same concentration adenosine 5'-triphosphate disodium (ATP) (p < 0.01). alpha,beta-Methylene-ATP (alpha,beta-meATP)-activated currents in DRG neurons of CCI rats were more obvious than those obtained in other group rats (p < 0.01). The results showed that TMP may inhibit the primary afferent transmission of neuropathic pain induced by P2X(3) receptor. (c) 2008 Elsevier Inc. All rights reserved.