Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii
Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii
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DOI：
10.1016/j.ymben.2016.07.013
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发表时间：
2016-11-01
影响因子：
8.4
通讯作者：
Kruse, Olaf
中科院分区：
文献类型：
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作者：
Lauersen, Kyle J.;Baier, Thomas;Kruse, Olaf
萜烯合成酶在微生物宿主体内的异源表达为生物生产所需代谢物开辟了许多可能性。光合微生物宿主提供了传统发酵系统的可持续替代方案，利用可自由获得的（太阳）光和二氧化碳作为生物生产的投入。在这里，我们报道了一种广藿香酚合成酶在模型绿色微藻莱茵衣藻中的表达。从海藻中提取倍半萜类化合物广藿香醇，并将其作为倍半萜类化合物生产能力的标志。采用一种新的基因加载策略，在6天内产生了高达922 +/- 242 μ g(-1) CDW的广藿香醇。此外，我们还研究了碳源对大规模批量栽培中倍半萜类化合物产量的影响。结果表明，在完全光自养条件下，海藻可产生高达1.03 mg L-1的倍半萜类产物，并且与碳源有关的倍半萜类产物代谢发生了变化。(C) 2016国际代谢工程学会。Elsevier Inc.出版。版权所有。
The heterologous expression of terpene synthases in microbial hosts has opened numerous possibilities for bioproduction of desirable metabolites. Photosynthetic microbial hosts present a sustainable alternative to traditional fermentative systems, using freely available (sun)light and carbon dioxide as inputs for bio-production. Here, we report the expression of a patchoulol synthase from Pogostemon cablin Benth in the model green microalga Chlamydomonas reinhardtii. The sesquiterpenoid patchoulol was produced from the alga and was used as a marker of sesquiterpenoid production capacity. A novel strategy for gene loading was employed and patchoulol was produced up to 922 +/- 242 mu g g(-1) CDW in six days. We additionally investigated the effect of carbon source on sesquiterpenoid productivity from C. reinhardtii in scale-up batch cultivations. It was determined that up to 1.03 mg L-1 sesquiterpenoid products could be produced in completely photoautotrophic conditions and that the alga exhibited altered sesquiterpenoid production metabolism related to carbon source. (C)) 2016 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.