A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae
A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae
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DOI：
10.1073/pnas.012602299
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发表时间：
2002-01-22
影响因子：
11.1
通讯作者：
Mekalanos, JJ
中科院分区：
文献类型：
--
作者：
Akerley, BJ;Rubin, EJ;Mekalanos, JJ
对流感嗜血杆菌基因组应用了一种高密度转座子诱变策略，以鉴定生长或存活所需的基因。该分析检测到259个功能未知的开放阅读框（ORF）产物具有假定的关键作用。对完整基因组的比较确定了流感嗜血杆菌中这些蛋白质的一个子集，它们在结核分枝杆菌中有同源物，但在酿酒酵母中不存在，这种分布模式有利于将它们用于开发抗菌疗法。该子集中的三个基因对其他细菌的存活至关重要。将流感嗜血杆菌中的关键基因产物集合与其他微生物中的同源物分布相结合，可以检测到多种细菌中未被识别的关键细胞通路的组成部分。这种基因组规模的表型分析确定了大量功能未知基因的潜在作用。
A high-density transposon mutagenesis strategy was applied to the Haemophilus influenzae genome to identify genes required for growth or viability. This analysis detected putative essential roles for the products of 259 ORFs of unknown function. Comparisons between complete genomes defined a subset of these proteins in H. influenzae having homologs in Mycobacterium tuberculosis that are absent in Saccharomyces cerevisiae, a distribution pattern that favors their use in development of antimicrobial therapeutics. Three genes within this set are essential for viability in other bacteria. Interfacing the set of essential gene products in H. influenzae with the distribution of homologs in other microorganisms can detect components of unrecognized cellular pathways essential in diverse bacteria. This genome-scale phenotypic analysis identifies potential roles for a large set of genes of unknown function.