Importance of species-specific antigens in the serodiagnosis of Chlamydia trachomatis reactive arthritis
Importance of species-specific antigens in the serodiagnosis of Chlamydia trachomatis reactive arthritis
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DOI：
10.1093/rheumatology/41.9.1017
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发表时间：
2002-09-01
期刊：
影响因子：
5.5
通讯作者：
Vischer, TL
中科院分区：
文献类型：
--
作者：
Bas, S;Genevay, S;Vischer, TL
目标。确定用于沙眼衣原体反应性关节炎血清诊断的最灵敏和特异的抗衣原体抗体测量方法。方法。使用免疫印迹、使用六种合成肽或重组抗原的酶联免疫吸附测定以及微量免疫荧光测试来确定 17 名沙眼衣原体反应性关节炎患者的血清样本中是否存在 IgG、IgM 和 IgA。将 20 名患有其他炎症性关节病但没有泌尿生殖道沙眼衣原体感染证据的患者用作对照。结果。当确定 IgG 和/或 IgA 对两种物种特异性抗原的反应性时，获得了灵敏度 (76%) 和特异性 (85%) 的最佳关联。这些抗原是合成肽，源自主要外膜蛋白 (MOMP) 可变结构域 IV 中的物种特异性表位（Labsystems，芬兰）和由质粒开放阅读框 3 (pgp3) 编码的重组多肽。结论。 IgG 和/或 IgA 抗 MOMP 衍生肽和抗 pgp3 可用于诊断可能的沙眼衣原体反应性关节炎。
Objectives. To determine the most sensitive and specific method of anti-Chlamydia antibody measurement for the serodiagnosis of Chlamydia trachomatis reactive arthritis.Methods. Immunoblotting, enzyme-linked immunosorbent assays using six synthetic peptides or recombinant antigens and a microimmunofluorescence test were used to determine the presence of IgG, IgM and IgA in serum samples from 17 patients with C. trachomatis reactive arthritis. Twenty patients with other inflammatory arthropathies without evidence of urogenital C. trachomatis infection were used as controls.Results. The best association of sensitivity (76%) and specificity (85%) was obtained when IgG and/or IgA reactivity to two species-specific antigens was determined. These antigens were synthetic peptides, derived from species-specific epitopes in the variable domain IV of the major outer membrane protein (MOMP) (Labsystems, Finland) and recombinant polypeptide encoded by open reading frame 3 of the plasmid (pgp3).Conclusions. IgG and/or IgA anti-MOMP-derived peptides and anti-pgp3 could be useful for the diagnosis of probable C. trachomatis reactive arthritis.