Stress-strain measurements and viscoelastic response of blood vessels cryopreserved by vitrification
Stress-strain measurements and viscoelastic response of blood vessels cryopreserved by vitrification
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DOI：
10.1007/s10439-007-9372-0
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发表时间：
2007-12-01
影响因子：
3.8
通讯作者：
Rabin, Yoed
中科院分区：
文献类型：
--
作者：
Rios, Jorge L. Jimenez;Steif, Paul S.;Rabin, Yoed
为了获得更多的了解在低温保存过程中的热机械现象，拉伸应力松弛实验进行玻璃化血管（玻璃体在拉丁语中的意思是玻璃），并与各种粘弹性模型的结果进行比较。使用最近提出的设备，等温应力松弛的结果，获得了牛颈动脉模型，渗透与冷冻保护剂鸡尾酒VS 55和参考溶液的7.05 M DMSO。在快速施加拉伸应变后，实验结果显示应力随时间呈指数衰减;在给定时间的应力随着试样温度的降低而增加。在所研究的粘弹性模型中，Williams-Landel-Ferry模型被发现最适合应力松弛数据随温度的变化，而Maxwell-Weichert模型被用来表示应力随时间的指数衰减。发现血管性质在高于约-100 ° C的温度下占主导地位，而冷冻保护剂的性质在低于该温度下占主导地位。当相对于冷冻保护剂玻璃化转变温度归一化时，适当定义的稳态粘度显示出两种冷冻保护剂的相似行为。
To gain increased insight into thermo-mechanical phenomena during cryopreservation, tensile stress relaxation experiments were conducted on vitrified blood vessels (vitreous in Latin means Glassy), and the results compared with various viscoelastic models. Using a recently presented device, isothermal stress-relaxation results were obtained for a bovine carotid artery model, permeated with the cryoprotectant cocktail VS55 and a reference solution of 7.05 M DMSO. After a rapidly applied tensile strain, experimental results display exponential decay of stress with time; the stress at a given time increases with decreasing specimen temperature. Among the viscoelastic models investigated, the Williams-Landel-Ferry model was found to best-fit the variation of the stress relaxation data with temperature, while a Maxwell-Weichert model is used to represent the exponential decay of the stress with time. Blood vessel properties were found to dominate at temperatures above roughly -100 degrees C, while the properties of the cryoprotectant dominate below this temperature. A suitably defined steady-state viscosity displayed a similar behavior for both cryoprotectants, when normalized with respect to the cryoprotectant glass transition temperature.