Self-Organized Synchronous Calcium Transients in a Cultured Human Neural Network Derived from Cerebral Organoids
Self-Organized Synchronous Calcium Transients in a Cultured Human Neural Network Derived from Cerebral Organoids
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DOI：
10.1016/j.stemcr.2019.05.029
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发表时间：
2019-09-10
影响因子：
5.9
通讯作者：
Takahashi, Jun
中科院分区：
文献类型：
--
作者：
Sakaguchi, Hideya;Ozaki, Yuki;Takahashi, Jun
大脑是大脑功能的主要中心，其活动来源于神经网络中激活细胞的组装。复杂的人脑神经元网络活动的研究是目前比较困难的。在这里，使用大脑类器官，我们报告了自组织和复杂的人类神经网络活动，包括同步和非同步模式。在人胚胎干细胞衍生的脑类器官的解离培养后观察到自组织神经元网络形成。通过钙成像测量网络的自发个体和同步活动，随后的分析能够检查详细的细胞活动模式，提供同步光栅图，聚类分析和细胞分布数据。最后，我们证明了我们的系统来评估药物诱导的网络活动的动态变化的可行性。利用该系统对人类神经元网络进行全面的功能分析，可以为深入了解人类大脑功能提供有力的工具。
The cerebrum is a major center for brain function, and its activity is derived from the assembly of activated cells in neural networks. It is currently difficult to study complex human cerebral neuronal network activity. Here, using cerebral organoids, we report self-organized and complex human neural network activities that include synchronized and non-synchronized patterns. Self-organized neuronal network formation was observed following a dissociation culture of human embryonic stem cell-derived cerebral organoids. The spontaneous individual and synchronized activity of the network was measured via calcium imaging, and subsequent analysis enabled the examination of detailed cell activity patterns, providing simultaneous raster plots, cluster analyses, and cell distribution data. Finally, we demonstrated the feasibility of our system to assess drug-inducible dynamic changes of the network activity. The comprehensive functional analysis of human neuronal networks using this system may offer a powerful tool to access human brain function.