Vitamin D inhibits myometrial and leiomyoma cell proliferation in vitro
Vitamin D inhibits myometrial and leiomyoma cell proliferation in vitro
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DOI：
10.1016/j.fertnstert.2008.02.136
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发表时间：
2009-05-01
影响因子：
6.7
通讯作者：
Heinonen, Pentti K.
中科院分区：
文献类型：
--
作者：
Blauer, Merja;Rovio, Paivi H.;Heinonen, Pentti K.
目的：确定1，25（OH）（2）D-3和25（OH）D3维生素D衍生物在体外对子宫平滑肌瘤和子宫肌层细胞生长的影响。设计：体外研究。设置：细胞生物学研究实验室。患者：6名绝经前子宫平滑肌瘤妇女接受子宫切除术。干预：采集平滑肌瘤和正常子宫肌层组织样本，建立配对培养。主要结果测量：结果：0.1nM生理浓度的1，25（OH）（2）D-3对子宫肌层细胞和子宫平滑肌瘤细胞的生长抑制率为12%。生长抑制具有浓度依赖性;最高浓度的1，25（OH）（2）D-3（100 nM）对两种细胞类型的生长均抑制62%。所有差异均具有统计学意义。一个小小的刺激（
Objective: To determine the effect of 1,25(OH)(2)D-3 and 25(OH)D3 vitamin D derivates on the growth of leiomyoma and myometrial cells in vitro.Design: In vitro study.Setting: Cell biology research laboratory.Patient(s): Six premenopausal women with uterine leiomyomas undergoing hysterectomy.Intervention(s): Samples of leiomyomas and normal myometrial tissue were obtained, and paired cultures were established.Main Outcome Measure(s): A colorimetric crystal violet assay to determine the effect of 1,25(OH)2D3 and 25(OH)D3 on cell growth.Result(s): In both myometrial and leiomyoma cells, 0.1 nM physiologic level of 1,25(OH)(2)D-3 inhibited growth by 12% when compared with controls. The growth inhibition was concentration dependent; the highest concentration of 1,25(OH)(2)D-3 (100 nM) inhibited growth by 62% in both cell types. All the differences were statistically significant. A slight stimulation (