Spatial and temporal variability in the potential of river water biofilms to degrade p-nitrophenol.
Spatial and temporal variability in the potential of river water biofilms to degrade p-nitrophenol.
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DOI：
10.1016/j.chemosphere.2016.08.095
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发表时间：
2016-12
期刊：
影响因子：
8.8
通讯作者：
A. Kowalczyk;O. Price;C. J. van der Gast;C. Finnegan;R. V. van Egmond;H. Schäfer;G. Bending
中科院分区：
文献类型：
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作者：
A. Kowalczyk;O. Price;C. J. van der Gast;C. Finnegan;R. V. van Egmond;H. Schäfer;G. Bending
为了预测化学物质在环境中的命运，使用从环境隔间收集的微生物接种物进行了一系列监管测试，以研究生物降解的潜力。环境中微生物的丰度和分布受到一系列变量的影响，因此生物降解试验中使用的接种物的多样性和生物量在空间和时间上可能存在很大差异。在监管测试中使用人工或天然生物膜可以使更一致的微生物群落用作接种物，以提高测试的一致性。我们研究了河水中形成的细菌生物膜的成分、生物量和化学生物降解潜力的时空变化。采样时间和采样位置影响生物膜降解对硝基苯酚 (PNP) 的能力。生物膜细菌群落结构随采样时间的不同而变化，但不受采样位置的影响。 PNP 的降解与丁香假单胞菌相对丰度的增加有关。将细菌元群落划分为核心类群和卫星类群表明，PNP 的降解与丁香假单胞菌相对丰度的增加有关。 注射器可能是整个采样时间内群落的卫星成员或核心成员，但这对 PNP 降解没有影响。 pnpA 基因的定量 PCR 分析表明，无论样品降解 PNP 的能力如何，它都存在于所有样品中。河流生物膜的生物量、微生物群落组成和PNP生物降解潜力存在季节性变化，导致生物降解测试结果不一致。我们在监管化学降解测试变化的潜在机制的背景下讨论结果。
In order to predict the fate of chemicals in the environment, a range of regulatory tests are performed with microbial inocula collected from environmental compartments to investigate the potential for biodegradation. The abundance and distribution of microbes in the environment is affected by a range of variables, hence diversity and biomass of inocula used in biodegradation tests can be highly variable in space and time. The use of artificial or natural biofilms in regulatory tests could enable more consistent microbial communities be used as inocula, in order to increase test consistency. We investigated spatial and temporal variation in composition, biomass and chemical biodegradation potential of bacterial biofilms formed in river water. Sampling time and sampling location impacted the capacity of biofilms to degradep-nitrophenol (PNP). Biofilm bacterial community structure varied across sampling times, but was not affected by sampling location. Degradation of PNP was associated with increased relative abundance ofPseudomonas syringae.Partitioning of the bacterial metacommunity into core and satellite taxa revealed that theP. syringaecould be either a satellite or core member of the community across sampling times, but this had no impact on PNP degradation. Quantitative PCR analysis of thepnpAgene showed that it was present in all samples irrespective of their ability to degrade PNP. River biofilms showed seasonal variation in biomass, microbial community composition and PNP biodegradation potential, which resulted in inconsistent biodegradation test results. We discuss the results in the context of the mechanisms underlying variation in regulatory chemical degradation tests.