RNA interference for improving the outcome of islet transplantation.
RNA interference for improving the outcome of islet transplantation.
复制标题
DOI：
10.1016/j.addr.2010.11.003
复制
发表时间：
2011-01
影响因子：
16.1
通讯作者：
Mahato, Ram I.
中科院分区：
文献类型：
--
作者：
Li, Feng;Mahato, Ram I.
胰岛移植有治愈1型糖尿病的潜力。尽管最近的治疗取得了成功，但仍然不常见，因为大量移位的胰岛会受到多种挑战的破坏，包括即时血液介导的炎症反应、缺氧/再灌注损伤、炎性细胞因子和免疫排斥反应。RNA干扰(RNAi)是一种选择性降解靶基因的新策略。利用RNAi技术下调有害基因的表达有可能改善胰岛移植的结果。本综述的目的是彻底了解胰岛移植的生物学障碍，并讨论如何使用不同的RNAi技术克服这些障碍。这最终将有助于改善胰岛移植后的存活和功能。讨论了化学合成的小干扰RNA(SiRNA)、基于载体的短Haripin RNA(ShRNA)及其关键设计元素(如序列、启动子、骨架)。并对组合RNAi在胰岛移植中的应用进行了讨论。最后讨论了增强基因沉默的几种传递策略，包括siRNA的化学修饰、复合体形成、生物偶联和病毒载体。
Islet transplantation has the potential to cure type 1 diabetes. Despite recent therapeutic success, it is still not common because a large number of transpanted islets get damaged by multiple challenges including instant blood mediated inflammatory reaction, hypoxia/reperfusion injury, inflammatory cytokines, and immune rejection. RNA interference (RNAi) is an novel strategy to selectively degrade target mRNA. The use of RNAi technologies to downregulate the expression of harmful genes has the potential to improve the outcome of islet transplantation. The aim of this review is to gain a thorough understanding of biological obstacles to islet transplantation and discuss how to overcome these barriers using different RNAi technologies. This eventually will help improve islet survival and function post transplantaion. Chemically synthesized small interferring RNA (siRNA), vector based short haripin RNA (shRNA), and their critical design elements (such as sequences, promoters, backbone) are discussed. The application of combinatorial RNAi in islet transplantation is also discussed. Last but not the least, several delivery strategies for enhanced gene silencing are discussed, including chemical modification of siRNA, complex formation, bioconjugation, and viral vectors.
登录
查看更多内容
DOI：
10.1016/s0006-291x(02)02013-2
发表时间：
2002-08-30
影响因子：
3.1
作者：
Bertrand, JR;Pottier, M;Malvy, C
通讯作者：
Malvy, C
影响因子：
5.1
作者：
通讯作者：
--
影响因子：
2.7
作者：
Bouwman, LH;Ling, ZD;Roep, BO
通讯作者：
Roep, BO
影响因子：
3.5
作者：
Burkhardt, BR;Lyle, R;Zhang, YC
通讯作者：
Zhang, YC
影响因子：
56.9
作者：
BENDTZEN, K;MANDRUPPOULSEN, T;SVENSON, M
通讯作者：
SVENSON, M