Nano zero-valent iron improves anammox activity by promoting the activity of quorum sensing system.
Nano zero-valent iron improves anammox activity by promoting the activity of quorum sensing system.
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DOI：
10.1016/j.watres.2021.117491
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发表时间：
2021-08
期刊：
影响因子：
12.8
通讯作者：
Zhibin Wang;Xiaolin Liu;S. Ni;Xuming Zhuang;Taeho Lee
中科院分区：
文献类型：
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作者：
Zhibin Wang;Xiaolin Liu;S. Ni;Xuming Zhuang;Taeho Lee
纳米零价铁（nZVI）的添加已被证明可以提高厌氧氨氧化工艺的效率，但其作用机理尚不清楚。本文采用宏基因组测序方法研究了nZVI对厌氧氨氧化微生物群落的影响。结果表明，50 mg/L的nZVI确实促进了厌氧氨氧化反应器对NH 4+和NO2−的去除，并显著提高了厌氧氨氧化细菌（Ca. Brocadia）从42.1%降至52.5%。50 mg/L nZVI使微生物群落中c-di-GMP合成蛋白的丰度从148 rpmr增加到252 rpmr，使c-di-GMP降解蛋白的丰度从238 rpmr降低到204 rpmr，间接导致了c-di-GMP在微生物群落中的富集。c-di-GMP的富集降低了反应器中微生物的运动性，促进了细菌分泌胞外聚合物，有利于厌氧氨氧化反应器中污泥颗粒的形成。本研究阐明了纳米零价铁促进厌氧氨氧化的机理，为厌氧氨氧化的工程应用提供了理论指导。
The addition of nano zero-valent iron (nZVI) has been proven to improve the efficiency of the anammox process, however, the mechnism is not clear. Here, the effect of nZVI on anammox microbial community was studied by metagenomic sequencing methods. It was found that 50 mg/L nZVI indeed promoted the removal of NH4+and NO2−of the anammox reactor and significantly improved the relative abundance of AnAOB (Ca. Brocadia) from 42.1% to 52.5%. What's more, 50 mg/L nZVI increased the abundance of c-di-GMP synthesized protein from 148 rpmr to 252 rpmr in the microbial community and decreased the abundance of c-di-GMP degradation protein from 238 rpmr to 204 rpmr, which indirectly led to the enrichment of c-di-GMP in the microbial community. The enrichment of c-di-GMP reduced the motility of microorganisms in the reactor and promoted the secretion of extracellular polymers by bacteria, which is beneficial to the formation of sludge particles in the anammox reactor. In conclusion, this research clarified the mechanism of nZVI promoting the anammox process and provided theoretical guidance for the engineering application of anammox.