Complete assignment of heteronuclear protein resonances by protonless NMR spectroscopy
Complete assignment of heteronuclear protein resonances by protonless NMR spectroscopy
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DOI：
10.1002/anie.200461794
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发表时间：
2005-01-01
影响因子：
16.6
通讯作者：
Vasos, PR
中科院分区：
文献类型：
--
作者：
Bermel, W;Bertini, I;Vasos, PR
13 C的检测现在可以通过蛋白质的高分辨率核磁共振光谱来进行。在多维实验中用于13 C Homestrant解耦的协议与羰基、C和C核相关。这些技术还允许在专门的异源实验中检测侧链核，从而完成蛋白质的分配。
The detection of13C is now amenable to high-resolution NMR spectroscopy of proteins. A protocol for 13C homonuclear decoupling in multidimensional experiments correlates carbonyl, C , and C nuclei. These techniques also allow the detection of side-chain nuclei in exclusively heteronuclear experiments, and thus the complete assignment of proteins.