DNA microarray global gene expression analysis of influenza virus-infected chicken and duck cells.
DNA microarray global gene expression analysis of influenza virus-infected chicken and duck cells.
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DOI：
10.1016/j.gdata.2015.03.004
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发表时间：
2015-06
期刊：
影响因子：
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通讯作者：
Chang KC
中科院分区：
文献类型：
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作者：
Kuchipudi SV;Dunham SP;Chang KC
本文中描述的数据与Kuchipudi等人（2014）题为“鸡而非鸭的高致病性禽流感病毒感染与宿主免疫和促炎反应升高有关”的文章有关。鸡感染高致病性H5N1亚型禽流感病毒后，通常在1至2天内死亡率为100%，而鸭的感染则相反，导致轻微或无临床症状。鸡的致命性疾病的快速发作，但没有证据表明鸭的严重临床症状，表明其先天免疫机制的根本差异。本研究利用鸡基因芯片（Chicken Genechip Microarrays，Affytron）技术分析了低致病性禽流感（LPAI）H2N3病毒和两种HPAI H5N1亚型病毒感染的原代鸡和鸭肺细胞的基因表达谱，以了解鸡和鸭宿主易感性和抗性的分子基础。在这里，我们描述了对数据集进行的实验设计、质量控制和分析。这些数据可通过登录号为GSE 33389的基因表达综合数据库（GEO）公开获得，这些数据的分析和解释包括在Kuchipudi等人（2014）中。
The data described in this article pertain to the article by Kuchipudi et al. (2014) titled “Highly Pathogenic Avian Influenza Virus Infection in Chickens But Not Ducks Is Associated with Elevated Host Immune and Pro-inflammatory Responses”. While infection of chickens with highly pathogenic avian influenza (HPAI) H5N1 virus subtypes often leads to 100% mortality within 1 to 2 days, infection of ducks in contrast causes mild or no clinical signs. The rapid onset of fatal disease in chickens, but with no evidence of severe clinical symptoms in ducks, suggests underlying differences in their innate immune mechanisms. We used Chicken Genechip microarrays (Affymetrix) to analyse the gene expression profiles of primary chicken and duck lung cells infected with a low pathogenic avian influenza (LPAI) H2N3 virus and two HPAI H5N1 virus subtypes to understand the molecular basis of host susceptibility and resistance in chickens and ducks. Here, we described the experimental design, quality control and analysis that were performed on the data set. The data are publicly available through the Gene Expression Omnibus (GEO)database with accession number GSE33389, and the analysis and interpretation of these data are included in Kuchipudi et al. (2014).