In Vitro and In Vivo Evaluation of Titanium Surface Modification for Biological Aging by Electrolytic Reducing Ionic Water
In Vitro and In Vivo Evaluation of Titanium Surface Modification for Biological Aging by Electrolytic Reducing Ionic Water
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DOI：
10.3390/app9040713
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发表时间：
2019-02
期刊：
影响因子：
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通讯作者：
T. Miki;T. Matsuno;Y. Hashimoto;A. Miyake;T. Satomi
中科院分区：
文献类型：
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作者：
T. Miki;T. Matsuno;Y. Hashimoto;A. Miyake;T. Satomi
本研究利用电解还原离子水(S-100®)，对一种安全、简便的表面处理方法进行了体内外评价。对Ti-6Al-4V盘片进行清洗，并将盘片放置在干净的长凳上1周和4周进行老化。将这些圆盘分别浸泡在S-100®(S-100组)、超纯水(对照组)或紫外线照射(UV组)中，进行表面分析、细胞实验和动物实验。S-100组钛表面亲水性增强，体外蛋白质吸附量和细胞粘附率均有所提高。在活体中，观察到椎间盘周围有新的骨形成。这些发现表明，S-100®表面处理增加了生物老化钛表面的生物活性。我们正计划对其进行进一步的研究，为临床应用积累证据。
In this study, using electrolytic reducing ionic water (S-100®), a novel surface treatment method safely and easily modifying the surface properties was evaluated in vitro and in vivo. Ti-6Al-4V disks were washed and the disks were kept standing on a clean bench for one and four weeks for aging. These disks were immersed in S-100® (S-100 group), immersed in ultra-pure water (Control group), or irradiated with ultraviolet light (UV group), and surface analysis, cell experiment, and animal experiment were performed using these disks. The titanium surface became hydrophilic in the S-100 group and the amount of protein adsorption and cell adhesion rate were improved in vitro. In vivo, new bone formation was noted around the disk. These findings suggested that surface treatment with S-100® adds bioactivity to the biologically aged titanium surface. We are planning to further investigate it and accumulate evidence for clinical application.