A heparin-functionalized woven stent graft for endovascular exclusion
A heparin-functionalized woven stent graft for endovascular exclusion
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用于血管内排除的肝素功能化编织覆膜支架
DOI：
10.1016/j.colsurfb.2019.04.027
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发表时间：
2019-08-01
影响因子：
5.8
通讯作者：
Li, Gang
中科院分区：
文献类型：
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作者：
Liu, Zekun;Zheng, Zhaozhu;Li, Gang
近年来，随着血管疾病的增多，研制一种能长期满足移植要求的抗闭塞覆膜支架具有重要意义。在本文中，我们描述了一种丝素蛋白（SF）/肝素功能化分叉支架移植物（BSG），采用纺织成型技术。基于无缝编织技术对BSG进行原型制作，并在蒸汽/空气处理下用SF负载的肝素对表面进行改性以提高其通畅性。考察了BSG的厚度、透水性、接触角、力学性能、体外释药和凝血时间等物理性能。结果表明，肝素改性可以提高BSG的凝血时间，其抗水渗透性能达到1.154 ± 0.854 mL/（cm（2）x min），而它们的厚度仅为0.085 +/- 0.004 mm。BSG的肝素释放表明，通过增加β-SF的片状结构和结晶度。体外培养的人血管平滑肌细胞的存活率和贴壁率表明，修饰的BSG能显著抑制HVSM细胞的增殖。肝素功能化的BSG具有良好的厚度、抗水渗透性和抗阻塞功能，在血管疾病的治疗中具有潜在的应用价值。
With the increase of vascular diseases in recent years, it is of importance to develop an anti-occlusion stent graft, which can meet the requirements of transplants for a long term. In this paper, we describe a silk fibroin (SF)/heparin-functionalized bifurcated stent graft (BSG) using textile forming technology. The BSGs were prototyped based on seamless weaving technology, and the surface was modified with SF-loaded heparin under steam/air treatment to improve their patency. The physical properties such as thickness, water permeability, contact angle, mechanical properties, and in vitro drug release and coagulation time of the BSGs were examined. The results showed that heparin modification can improve its coagulation time, and the water permeability resistance of the BSGs reached 1.154 +/- 0.854 mL/(cm(2) x min), while their thicknesses were just 0.085 +/- 0.004 mm. The heparin release of the BSGs showed that the release time was prolonged upon steam treatment by means of the increase in the beta-sheet structure and crystallinity of SF. The viability and attachment of human vascular smooth muscle (HVSM) cells cultured in the release of modified BSGs demonstrated that the modified BSGs could significantly inhibit the proliferation of HVSM cells. The heparin-functionalized BSG with satisfactory thickness, water permeability resistance and anti-occlusion function, which has potential applications in the treatment of vascular diseases.