Cost- and time-efficient gel electrophoresis for mini-gel systems
Cost- and time-efficient gel electrophoresis for mini-gel systems
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DOI：
10.1006/abio.1998.2667
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发表时间：
1998-06-15
影响因子：
2.9
通讯作者：
Barnes, JA
中科院分区：
文献类型：
--
作者：
Comes, AV;Barnes, JA
蛋白质电泳法因其快速、经济和易于使用而成为蛋白质鉴定的主要手段之一。迷你凝胶系统能够以与传统凝胶系统相似或更好的分辨率更快地分离蛋白质和核酸。本文介绍了在不影响所得凝胶的分辨率的情况下，降低凝胶电泳的成本和时间的一般方法。用Bio-Rad Mini-Protean II仪器(美国加州Bio-Rad实验室有限公司)按Laemmli方法进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(PAGE)
Electrophoresis of proteins is now one of the primary means of protein characterization because of its speed, economy, and ease of use (1). Mini-gel systems have enabled faster protein and nucleic acid separation with similar or better resolution than conventional gel systems. This paper presents general methods for reducing cost and time with respect to gel electrophoresis without compromising the resolution of the resultant gel. Sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) was carried out using a Bio-Rad Mini-Protean II apparatus (Bio-Rad Laboratories Ltd., CA) according to the method of Laemmli