Intravital multiphoton microscopy for dissecting cellular dynamics in arthritic inflammation and bone destruction.
Intravital multiphoton microscopy for dissecting cellular dynamics in arthritic inflammation and bone destruction.
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DOI：
10.1007/978-1-4939-0404-4_1
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发表时间：
2014
影响因子：
--
通讯作者：
J. Kikuta;M. Ishii
中科院分区：
文献类型：
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作者：
J. Kikuta;M. Ishii
破骨细胞是从单核巨噬细胞/单核细胞系造血前体分化而来的巨大骨吸收多核体。它们不仅在正常的骨稳态（重塑）中起关键作用，而且在骨破坏性疾病如骨质疏松症和类风湿性关节炎的发病机制中也起关键作用。然而，成熟破骨细胞的活性如何在体内调节仍不清楚。为了回答这个问题，我们最近开发了一种先进的成像系统，用活体多光子显微镜观察活骨组织。使用这个系统，我们成功地在活bones.We可视化成熟破骨细胞在活的骨髓和关节中，使用活体多光子显微镜骨吸收的详细方法描述。这种方法将有利于研究关节炎炎症和骨破坏的细胞动力学在体内，因此将是有用的评价新的抗骨吸收药物。
Osteoclasts are giant bone-resorbing polykaryons that differentiate from mononuclear macrophage/monocyte-lineage hematopoietic precursors. They play critical roles not only in normal bone homeostasis (remodeling) but also in the pathogenesis of bone-destructive disorders such as osteoporosis and rheumatoid arthritis. However, how the activity of mature osteoclasts is regulated in vivo remains unclear. To answer this question, we recently developed an advanced imaging system to visualize living bone tissues with intravital multiphoton microscopy. Using this system, we succeeded in visualization of mature osteoclasts in living bones.We herein describe the detailed methodology for visualizing bone resorption of mature osteoclasts in living bone marrow and joints using intravital multiphoton microscopy. This approach would be beneficial for studying the cellular dynamics in arthritic inflammation and bone destruction in vivo and would thus be useful for evaluating novel anti-bone-resorptive drugs.