Region and lamina-specific distribution of extracellular matrix proteoglycans, hyaluronan and tenascin-R in the mouse hippocampal formation
Region and lamina-specific distribution of extracellular matrix proteoglycans, hyaluronan and tenascin-R in the mouse hippocampal formation
复制标题
DOI：
10.1016/s0891-0618(03)00036-x
复制
发表时间：
2003-08-01
影响因子：
2.8
通讯作者：
Schachner, M
中科院分区：
文献类型：
--
作者：
Brückner, G;Grosche, J;Schachner, M
众所周知，在成人大脑中，细胞外基质具有区域特异性。我们的比较细胞化学研究集中在小鼠海马形成中主要细胞外基质成分的层状组织，包括海马本身的CA1， CA2和CA3区域，齿状回，骨下和骨下前。采用n -乙酰半乳糖胺结合紫藤凝集素、胶体铁染色和针对不同蛋白聚糖结构域的抗体检测硫酸软骨素蛋白聚糖相关成分，包括聚集蛋白的Cat-301和Cat-315表位，以及神经蛋白、brevican和磷酸蛋白聚糖。这些成分的分布模式与透明质酸和脑特异性细胞外基质糖蛋白tenascine - r的分布模式相关，后者已知是细胞外基质蛋白聚糖的配体。凝集素结合清楚地标记了中间神经元周围的细胞外基质的神经元周围网，它们优先位于所有区域的主细胞层内或附近。在海马区，CA2亚场在锥体细胞体周围的神经pil和东地层和辐射体的神经pil区显示出强烈的标记。在对软骨素蛋白多糖结构域、brevican和phosphaca进行免疫反应后，以及在检测透明质酸和tenascine - r后，也可以看到这些模式。此外，在所有区域的神经pil中还表达了特征性的层流和层流内模式。在齿状回中，brevican、phosphacan和tenascin-R的染色强度主要在中间分子层，Cat-315的染色强度主要在内侧分子层，而neurocan的免疫反应性在外侧分子层向海马裂隙方向增强。我们的研究结果表明，蛋白聚糖、透明质酸和腱蛋白r在海马形成的各个区域和层中表现出不同的共表达模式。这些主要成分的不均匀组成表明，细胞外基质特别适应海马内连接和传入纤维系统的功能域。(C) 2003 Elsevier Science B.V.版权所有
The extracellular matrix is known to show region-specific characteristics in the adult brain. Our comparative cytochemical study is focused on the laminar organisation of major extracellular matrix constituents in the murine hippocampal formation, including the regions CA1, CA2 and CA3 of the hippocampus proper, the dentate gyrus, the subiculum and the presubiculum. Components related to chondroitin sulphate proteoglycans were detected by N-acetylgalactosamine-binding Wisteria floribunda agglutinin, colloidal iron staining, and antibodies to different proteoglycan domains, including the Cat-301 and Cat-315 epitopes of aggrecan, as well as neurocan, brevican and phosphacan. The distribution patterns of these components were correlated with the patterns revealed for hyaluronan and the brain-specific extracellular matrix glycoprotein, tenascin-R, known to be ligands of extracellular matrix proteoglycans. Lectin binding clearly labelled perineuronal nets of the extracellular matrix around interneurons, which were preferentially located within or near the principal cell layers in all regions. In the hippocampus proper, the CA2 subfield showed an intense labelling of the neuropil around pyramidal cell bodies and the neuropil zones in the strata oriens and radiatum. These patterns were also seen after immunoreaction for chondroitin proteoglycan domains, brevican and phosphacan, as well as after detection of hyaluronan and tenascin-R. Characteristic laminar and intralaminar patterns were additionally expressed in the neuropil in all regions. In the dentate gyrus, the staining intensity for brevican, phosphacan and tenascin-R was predominant in the middle molecular layer, and for Cat-315 in the inner molecular layer, whereas immunoreactivity for neurocan increased within the outer molecular layer towards the hippocampal fissure. Our findings indicate that proteoglycans, hyaluronan and tenascin-R show differential patterns of co-expression in the individual regions and laminae of the hippocampal formation. The inhomogeneous composition of these major components suggests that the extracellular matrix is specifically adapted to the functional domains of intrahippocampal connections and afferent fibre systems. (C) 2003 Elsevier Science B.V. All rights reserved.